MYL5 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86279
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NAFKMLDPDGKGKINKEYIKRLLMSQADKMTAEEVDQMFQFASIDVAGNLDYKALSYVI
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit MYL5 Antibody - BSA Free (NBP1-86279) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MYL5 Antibody - BSA Free
![Western Blot: MYL5 Antibody [NBP1-86279]](https://resources.bio-techne.com/images/products/MYL5-Antibody-Western-Blot-NBP1-86279-img0006.jpg "Western Blot: MYL5 Antibody [NBP1-86279]")
Western Blot: MYL5 Antibody [NBP1-86279]
Western Blot: MYL5 Antibody [NBP1-86279] - Analysis in control (vector only transfected HEK293T lysate) and MYL5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: MYL5 Antibody [NBP1-86279]](https://resources.bio-techne.com/images/products/MYL5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86279-img0005.jpg "Immunocytochemistry/ Immunofluorescence: MYL5 Antibody [NBP1-86279]")
Immunocytochemistry/ Immunofluorescence: MYL5 Antibody [NBP1-86279]
Immunocytochemistry/Immunofluorescence: MYL5 Antibody [NBP1-86279] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.![Immunohistochemistry-Paraffin: MYL5 Antibody [NBP1-86279]](https://resources.bio-techne.com/images/products/MYL5-Antibody-Immunohistochemistry-Paraffin-NBP1-86279-img0002.jpg "Immunohistochemistry-Paraffin: MYL5 Antibody [NBP1-86279]")
Immunohistochemistry-Paraffin: MYL5 Antibody [NBP1-86279]
Immunohistochemistry-Paraffin: MYL5 Antibody [NBP1-86279] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.Applications for MYL5 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MYL5
Alternate Names
light polypeptide 5, regulatory, MYLC2, myLC-2, myosin light chain 5, Myosin regulatory light chain 5, myosin, light chain 5, regulatory, Superfast myosin regulatory light chain 2
Gene Symbol
MYL5
Additional MYL5 Products
Product Documents for MYL5 Antibody - BSA Free
Product Specific Notices for MYL5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...