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mtTFA Antibody (24GB4085)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50411

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NBP3-50411-100ul
NBP3-50411-20ul

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB4085

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human mtTFA (Uniprot: Q00059)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for mtTFA Antibody (24GB4085)

mtTFA Antibody (24GB4085)

Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (24GB4085)[NBP3-50411] -

Immunocytochemical staining of HT-1080 cells with mtTFA antibody (1:1,000). Nuclei were stained blue with DAPI; mtTFA was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
mtTFA Antibody (24GB4085)

Flow Cytometry: mtTFA Antibody (24GB4085)[NBP3-50411] -

Flow cytometric analysis of mtTFAexpression in HT-1080 cells using mtTFA antibody (1:2,000). Green, isotype control; red, TFAM.
mtTFA Antibody (24GB4085)

Western Blot: mtTFA Antibody (24GB4085)[NBP3-50411] -

Western blotting analysis using mtTFA antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for mtTFA Antibody (24GB4085)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: mtTFA

TFAM a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this protein is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of the gene was eliminated by targeted disruption in heart and muscle cells.

Alternate Names

mitochondrial transcription factor A, MTTF1, mtTFA, TCF-6, TCF6L1, TCF6L2Mitochondrial transcription factor 1, TCF6TCF6L3, Transcription factor 6, Transcription factor 6-like 2, Transcription factor 6-like 2 (mitochondrial transcription factor), transcription factor A, mitochondrial

Gene Symbol

TFAM

Additional mtTFA Products

Product Documents for mtTFA Antibody (24GB4085)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for mtTFA Antibody (24GB4085)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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