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MRPL9 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89561

Novus Biologicals, part of Bio-Techne
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NBP1-89561
NBP1-89561-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NAPDLACNFSLSQNRGTVIVERWWKVPLAGEGRKPRLHRRHRVYKLVEDTKHRPKENLELILTQSVENVGVRGDLVSVKKSLGRNRLLPQGLAVYASPENKKLFEEEKLLRQEGKLEKIQTKAGEATVKFLKSCRLEVGMKNNV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MRPL9 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MRPL9 Antibody [NBP1-89561]

Immunocytochemistry/ Immunofluorescence: MRPL9 Antibody [NBP1-89561]

Immunocytochemistry/Immunofluorescence: MRPL9 Antibody [NBP1-89561] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
MRPL9 Antibody - BSA Free Immunohistochemistry-Paraffin: MRPL9 Antibody - BSA Free [NBP1-89561]

Immunohistochemistry-Paraffin: MRPL9 Antibody - BSA Free [NBP1-89561]

Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
MRPL9 Antibody - BSA Free Western Blot: MRPL9 Antibody - BSA Free [NBP1-89561]

Western Blot: MRPL9 Antibody - BSA Free [NBP1-89561]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp

Applications for MRPL9 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MRPL9

MRPL9, also known as Mitochondrial Ribosomal Protein L9, consists of a 267 amino acid isoform that is 30 kDa, and is involved in the manufacture of proteins within the mitochondria. Current disease research is working to determine the relationship between MRPL9 and obesity. MRPL9 has been linked to the process of translation, where it interacts with CA12, ICT1, SAT1, SLX4, and MRPL11.

Alternate Names

L9mt, mitochondrial ribosomal protein L9, MRP-L9,39S ribosomal protein L9, mitochondrial

Gene Symbol

MRPL9

Additional MRPL9 Products

Product Documents for MRPL9 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MRPL9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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