Detects mouse Stella/Dppa3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human Stella is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Stella/Dppa3 Antibody

Stella/Dppa3 in mouse ovary tissue.

Stella/Dppa3 was detected in perfusion fixed frozen sections of mouse ovary tissue using Goat Anti-Mouse Stella/Dppa3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2566) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to oocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse Stella/Dppa3 Antibody

Application

Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse ovary

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Stella/Dppa3

Reviewed Applications

Read 2 reviews rated 4.5 using AF2566 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)
Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stella/Dppa3

Stella, also known as Dppa3 (Developmental pluripotency-associated 3) and PGC7 (primordial germ cell 7) is expressed in primordial germ cells, oocytes, preimplantation embryos, and pluripotent cells. Although the fuction of Stella is not known, the expression pattern of the protein suggests a role in the maintenance of cell pluripotency.

Long Name

Developmental Pluripotency-associated Protein 3

Alternate Names

Dppa3

Entrez Gene IDs

359787 (Human); 73708 (Mouse)

Gene Symbol

DPPA3

UniProt

Q8QZY3

Additional Stella/Dppa3 Products

Product Documents for Mouse Stella/Dppa3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Stella/Dppa3 Antibody

For research use only

Citations
Reviews

Mouse Stella/Dppa3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2566

Key Product Details

Species Reactivity

Validated:

Cited:

Applications