Mouse Siglec-1/CD169 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB5610P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 645608
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Siglec-1/CD169
Thr20-Leu1639 (predicted)
Accession # Q62230
Thr20-Leu1639 (predicted)
Accession # Q62230
Specificity
Detects mouse Siglec-1/CD169 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Siglec‑1/CD169, -5, -6, -7, -8, -9, -10, -11, -14, recombinant mouse Siglec-2, -3, -E, -F, -G, or -H is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Siglec-1/CD169 PE-conjugated Antibody
Detection of Siglec‑1/CD169 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS overnight were stained with Rat Anti-Mouse Siglec-1/CD169 PE-conjugated Monoclonal Antibody (Catalog # FAB5610P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse Siglec-1/CD169 PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS overnight
Sample: RAW 264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS overnight
Reviewed Applications
Read 1 review rated 5 using FAB5610P in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Siglec-1/CD169
Siglec-1, also known as Sialoadhesin or CD169, is a 175-185 kDa type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily. Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains. The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains. Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections. It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43.
Long Name
Sialic Acid Binding Ig-like Lectin 1
Alternate Names
CD169, Siglec1
Gene Symbol
SIGLEC1
UniProt
Additional Siglec-1/CD169 Products
Product Documents for Mouse Siglec-1/CD169 PE-conjugated Antibody
Product Specific Notices for Mouse Siglec-1/CD169 PE-conjugated Antibody
For research use only
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