Mouse/Rat SCF/c-kit Ligand Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6998U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant rat SCF/c-kit Ligand
Gln26-Ala189
Accession # P21581
Gln26-Ala189
Accession # P21581
Specificity
Detect mouse and rat SCF/c-kit Ligand in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human SCF/c-kit Ligand is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: SCF/c-kit Ligand
SCF (Stem Cell Factor; also kit ligand and MGF) is a 40-45 kDa member of the SCF family of molecules. It is expressed by multiple cell types, including Sertoli cells, neurons, select fibroblasts, mast cells, melanocytes and dendritic cells. SCF binds to SCFR/c-kit (for kitten; the animal used to isolate it), and occurs in both a soluble and membrane-bound form. The soluble form of SCF produces a transient activation of SCFR, while the membrane-bound form generates a long-lasting signal in the SCFR due to the ligation and retention of SCFR at the expressing cell surface. Mature rat SCF is a 248 amino acid (aa) type I transmembrane (TM) glycoprotein (aa 26-273). It contains a 189 aa extracellular region (aa 26-214) plus a 36 aa cytoplasmic domain (aa 238-273). Proteolytic cleavage after Ala190 generates a 25-38 kDa soluble form that circulates as both a monomer and noncovalent homodimer. There is also possible cleavage after Phe183 that will create a similarly-sized isoform. An alternative splice form also exists that contains a Gly substitution for aa 174-292. This 28-32 kDa TM form may also undergo cleavage to generate a soluble 19-21 kDa isoform. Over aa 26-189, rat SCF shares 94%, 77% and 79% aa sequence identity with mouse, feline and human SCF, respectively.
Long Name
Stem Cell Factor
Alternate Names
c-kit Ligand, DCUA, DFNA69, FPH2, FPHH, KITLG, KL-1, MGF, SHEP7, SLF
Gene Symbol
KITLG
UniProt
Additional SCF/c-kit Ligand Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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