Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody

Detection of Proprotein Convertase 9/PCSK9 by Western Blot

Fishmeal feeding leads to circulating PCSK9. Expression of hepatic genes important for steroid hormone synthesis and cholesterol metabolism in mice fed fishmeal, salmon filet or beef (n = 10). a Validation RNA sequencing analysis with RT-qPCR analysis of selected genes identified as low expressed in fishmeal compared to salmon or beef (Hmgcr, Pcsk9, Fabp5, Fdsp, Chrna4). b Expression of mRNAs encoding genes regulating cholesterol synthesis (Srebf2) and lipogenesis (Srebf1c and Mlxipl/Chrebp). c Expression of cholesterol metabolite sensors (Nr1h3/LXR alpha and Nr1h2/LXR beta), cholesterol efflux transporters (Abca1 and Abcg1) and the rate-limiting enzyme in bile acid synthesis (Cyp7a1). d Plasma PCSK9 protein levels. e–f Immunoblot analysis and relative quantification of hepatic Pcsk9, Ldlr, Hmgcr and Gapdh protein levels (n = 5). Statistical testing was done with one-way ANOVA and Tukey test for multiple comparisons. $p < 0.05 between chow and at least two other groups. *p < 0.05, *p < 0.01 between indicated groups Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35788891), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Proprotein Convertase 9/PCSK9 by Western Blot

Fishmeal feeding leads to circulating PCSK9. Expression of hepatic genes important for steroid hormone synthesis and cholesterol metabolism in mice fed fishmeal, salmon filet or beef (n = 10). a Validation RNA sequencing analysis with RT-qPCR analysis of selected genes identified as low expressed in fishmeal compared to salmon or beef (Hmgcr, Pcsk9, Fabp5, Fdsp, Chrna4). b Expression of mRNAs encoding genes regulating cholesterol synthesis (Srebf2) and lipogenesis (Srebf1c and Mlxipl/Chrebp). c Expression of cholesterol metabolite sensors (Nr1h3/LXR alpha and Nr1h2/LXR beta), cholesterol efflux transporters (Abca1 and Abcg1) and the rate-limiting enzyme in bile acid synthesis (Cyp7a1). d Plasma PCSK9 protein levels. e–f Immunoblot analysis and relative quantification of hepatic Pcsk9, Ldlr, Hmgcr and Gapdh protein levels (n = 5). Statistical testing was done with one-way ANOVA and Tukey test for multiple comparisons. $p < 0.05 between chow and at least two other groups. *p < 0.05, *p < 0.01 between indicated groups Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35788891), licensed under a CC-BY license. Not internally tested by R&D Systems.