Mouse/Rat Kallikrein 7 Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7688U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Kallikrein 7
Gln22-Arg249 (predicted)
Accession # Q91VE3
Gln22-Arg249 (predicted)
Accession # Q91VE3
Specificity
Detects mouse and rat Kallikrein 7 in Western blots. Detects mouse Kallikrein 7 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Kallikrein 7 is observed, and less than 1% cross-reactivity with recombinant mous
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Kallikrein 7
KLK7 (Kallikrein 7; also Serine protease 6, Thymopsin and Stratum corneum chymotryptic enzyme/SCCE) is a 27 kDa (predicted), secreted glycoprotein member of the kallikrein subfamily, peptidase S1 family of enzymes. It is an IL-4 and IL-13-inducible chymotrypsin-like serine protease that has restricted expression, being associated with stratum spinosum and granulosum keratinocytes, distal and proximal convoluted tubule epithelium, limited pancreatic acinar cells. In contrast, KLK7 is widely expressed by a variety of carcinoma cell types. KLK7 preferentially cleaves peptide bonds that contain an aromatic (Tyr or Phe) residue in the P1 (or postcleavage C-terminal amino acid) position. Molecules known to serve as substrates for KLK7 include chemerin, fibronectin, E-cadherin, LL37, procaspase-14, MMP-9 and desmoglein. The nature of the KLK7 substrates indicates that it plays a key role in intercellular adhesion dissolution with subsequent cell migration. Mouse KLK7 is synthesized as a 249 amino acid (aa) precursor. It contains a 21 aa signal sequence, a four aa prosegment, and a 224 aa mature region (aa 26-249). The mature molecule contains one large protease domain (aa 26-246) and a variable, three aa C-terminus that possesses either a VSW or ASR motif. Over aa 22-249, mouse KLK7 shares 75% and 85% aa sequence identity with human and hamster KLK7, respectively.
Alternate Names
KLK7
Gene Symbol
KLK7
UniProt
Additional Kallikrein 7 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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