Western blot shows lysates of C2C12 mouse myoblast cell line, A20 mouse B cell lymphoma cell line, NRK rat normal kidney cells, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse/Rat HO‑1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3169) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Detection of Rat HO-1/HMOX1/HSP32 by Western Blot

Effect of prodigiosins on the mRNA and protein expression of HO-1 in gastric tissue.Data (means ± SEM of three assays) were normalized to the levels of GAPDH and expressed as fold induction (log2 scale), relative to the level of HO-1 in the control. aP < 0.05, significant difference vs. the health rats; bP < 0.05, significant difference vs. HCl/ethanol-induced rats, Duncan's post-hoc test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31194753), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse/Rat HO-1/HMOX1/HSP32 Antibody

Application

Recommended Usage

Western Blot

0.5 µg/mL
Sample: C2C12 mouse myoblast cell line, A20 mouse B cell lymphoma cell line, NRK rat normal kidney cells, and Rat-2 rat embryonic fibroblast cell line

Reviewed Applications

Read 2 reviews rated 4 using AF3169 in the following applications:

Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HO-1/HMOX1/HSP32

Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 (HO-1/HMOX1) and a constitutive heme oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Rat HO-1 shares 93% amino acid sequence identity with mouse HO-1.

Long Name

Heme Oxygenase 1

Alternate Names

HMOX1, HO1, HSP32

Entrez Gene IDs

3162 (Human); 24451 (Mouse); 15368 (Rat)

Gene Symbol

HMOX1

UniProt

P06762

Additional HO-1/HMOX1/HSP32 Products

Product Documents for Mouse/Rat HO-1/HMOX1/HSP32 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse/Rat HO-1/HMOX1/HSP32 Antibody

For research use only

Citations
Reviews

Mouse/Rat HO-1/HMOX1/HSP32 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3169

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse/Rat HO-1/HMOX1/HSP32 Antibody

Detection of Mouse/Rat HO-1/HMOX1/HSP32 by Western Blot.