Mouse/Rat DDT Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8354G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse DDT
Pro2-Leu118
Accession # O35215
Pro2-Leu118
Accession # O35215
Specificity
Detects mouse and rat DDT in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Mouse/Rat DDT Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: DDT
Mouse DDT (D-dopachrome tautomerase; also D-dopachrome decarboxylase and Phenylpyruvate tautomerase) is a 13 kDa member of the carboxy-lyases family. The DDT gene in mouse, rat and human is identical in exon structure to the Macrophage migration inhibitory factor (MIF) gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure. The genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10. DDT mRNA levels in human adipocytes are negatively correlated with obesity. The study on DDT knock-down adipocytes shows an increase in the expression of genes involved in both lipolysis and lipogenesis. DDT binds CD74 with high affinity, leading to activation of ERK1/2 MAP kinase and downstream pro-inflammatory pathways. Circulating levels of DDT correlate with disease severity in sepsis or malignancy. DDT also inhibits chemotaxis induced by CCL2 in macrophages. DDT in tumor angiogenesis promotes the expression of pro-angiogenic factors CXCL8 (IL8) and VEGF-A in non-small cell lung carcinoma cells through the CD74 receptor. Over aa 2-118, mouse DDT shares 95.7% and 73.5% aa identity with rat and human DDT, respectively.
Long Name
D-Dopachrome Tautomerase
Alternate Names
D-Dopachrome Decarboxylase, DDCT, DOPD, MIF2, Phenylpyruvate Tautomerase II
Gene Symbol
DDT
UniProt
Additional DDT Products
Product Specific Notices for Mouse/Rat DDT Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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