Species Reactivity:

Mouse

Immunogen:

Mouse myeloma cell line NS0-derived recombinant mouse NG2/MCSP
Leu1587-Ser2229
Accession # Q8VHY0

Applications:

Immunohistochemistry

Label:

Alexa Fluor 647 (Excitation=650 nm, Emission=668 nm)

Specificity:

Detects mouse NG2/MCSP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human NG2/MCSP/CSPG4 is observed.

View Full Technical Details for Mouse NG2/MCSP Alexa Fluor® 647-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: NG2/MCSP

NG2, also called MCSP (melanoma-associated chondroitin sulfate proteoglycan), CSPG4, or AN2, is a 400-500 kDa proteoglycan found on multiple cell types, including immature oligodendrocytes, osteoblasts, chondroblasts, and pericytes. It interacts with integrin alpha 3 beta 1, galectin 3, MMP16, collagen VI and PDGFR alpha. Mature mouse NG2 is a 2298 amino acid (aa) type I transmembrane glycoprotein. The extracellular region is 2200 aa in length (aa 30-2229), while the cytoplasmic domain is 76 aa in size. The glycoprotein component of NG2 is approximately 300-315 kDa in size. Proteolytic cleavage generates a 290 and 275 kDa form (excluding the chondroitin sulfate moiety). There are two splice variants. One shows a 38 aa substitution for aa 1657-2327, while another shows an alternate start site at Met1667. Over aa 31-420, mouse NG2 shares 95% and 80% aa identity with rat and human NG2, respectively.

Item	Value
Long Name	Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
Entrez Gene IDs	1464 (Human); 121021 (Mouse); 81651 (Rat)
Alternate Names	AN2, CSPG4, EC 3.6.3, HMW-MAA, MCSP, MCSPChondroitin sulfate proteoglycan NG2, MCSPG, MCSPGMelanoma chondroitin sulfate proteoglycan, MEL-CSPG, MSK16Melanoma-associated chondroitin sulfate proteoglycan, NG2, NG2EC 2.7.8, chondroitin sulfate proteoglycan 4, chondroitin sulfate proteoglycan 4 (melanoma-associated)

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Mouse NG2/MCSP Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6689R

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