Mouse MESDC2 Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4545G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MESDC2
Ala30-Leu224
Accession # Q9ERE7
Ala30-Leu224
Accession # Q9ERE7
Specificity
Detects mouse MESDC2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with human MESDC2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse MESDC2 Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: MESDC2
Mesoderm development candidate gene 2 (MESDC2, Mesd), also known as Boca in drosophila, is a 22 kDa protein that is required for formation of the primitive streak and mesoderm during embryogenesis (1-3). Mature mouse MESDC2 consists of an 83 amino acid (aa) structured central domain with flexible N- and C-terminal regions (4, 5). It shares 89% and 96% aa sequence identity with human and rat MESDC2, respectively. Although Boca lacks 34 aa in the C-terminal region and is only 40% identical with mouse MESDC2, the mouse protein can functionally substitute for Boca in drosophila S2 cells (6). A C-terminal ER retention motif localizes MESDC2 and Boca to the lumen of the endoplasmic reticulum (2, 6, 7). Within the ER, MESDC2 binds to the Wnt co-receptors LRP5 and LRP6 and is required for their proper folding and cell surface expression (2, 5, 8, 9). MESDC2 is therefore important for cellular Wnt responsiveness (9). When added extracellularly, MESDC2 binds to cell surface LRP6, preventing its interaction with the Wnt antagonist Dkk-1. This binding does not, however, trigger LRP6 internalization or alteration of cytoplasmic beta-catenin levels (8). An LRP5 mutant associated with high bone mass does not interact with MESDC2 (10). MESDC2 itself can be disrupted by a chromosomal translocation occurring in the germ cell tumor, infantile sacrococcygeal teratoma (11).
Long Name
Mesoderm Development Candidate 2
Alternate Names
BOCA
Entrez Gene IDs
23184 (Human)
Gene Symbol
MESD
UniProt
Additional MESDC2 Products
Product Specific Notices for Mouse MESDC2 Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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