Laminin alpha1/ beta1 was detected in immersion fixed frozen sections of embryonic mouse decidua (E10.5) using 10 µg/mL Rat Anti-Mouse Laminin alpha1/ beta1 Monoclonal Antibody (Catalog # MAB4656) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Laminin alpha1/ beta1 in Mouse Kidney, Skin, Intestine, and Skeletal Muscle.

Laminin alpha1/ beta1 was detected in perfusion fixed frozen sections of mouse kidney glomeruli (panel A), skin (panel B), intestine (panel C), and skeletal muscle (panel D) using Rat Anti-Mouse Laminin alpha1/ beta1 Monoclonal Antibody (Catalog # MAB4656) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse Laminin alpha1 / beta1 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse decidua (E10.5), and perfusion fixed frozen sections of mouse kidney glomeruli, skin, intestine, and skeletal muscle

Western Blot

Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349. This application was not tested by R&D Systems.

Reviewed Applications

Read 4 reviews rated 4.5 using MAB4656 in the following applications:

Immunocytochemistry/Immunofluorescence (3 Reviews)
Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin alpha 1/beta 1

Laminins are heterotrimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. Through interactions with integrins, dystroglycan, and other receptors, Laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes, and survival. Laminin-1 is comprised of alpha1, beta1, and gamma1 subunits.

References

Skubitz, A.P. et al. (1988) J. Biol. Chem. 263:4861.
Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349.

Additional Laminin alpha 1/beta 1 Products

Product Documents for Mouse Laminin alpha1 / beta1 Antibody

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COA

SDS

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Product Specific Notices for Mouse Laminin alpha1 / beta1 Antibody

For research use only

Citations
Reviews

Mouse Laminin alpha1 / beta1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4656

Key Product Details

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Validated:

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Label

Antibody Source

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Product Specifications

Immunogen

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Scientific Data Images for Mouse Laminin alpha1 / beta1 Antibody

Laminin alpha1/ beta1 in Embryonic Mouse Decidua.