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Mouse LAG-3 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB33281P

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FAB33281P-100UG
Conjugate
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 631501

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with mouse LAG-3
Ser23-Leu442
Accession # Q61790

Specificity

Detects mouse LAG-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LAG-3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse LAG-3 PE-conjugated Antibody

Detection of LAG-3 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of LAG-3 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes treated with PMA and Calcium Ionomycin for 3 days were stained with Rat Anti-Mouse LAG-3 PE-conjugated Monoclonal Antibody (Catalog # FAB33281P, filled histogram) or isotype control antibody (IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of LAG-3 in CTLL-2 mouse cytotoxic T cell line by Flow Cytometry

CTLL-2 mouse cytotoxic T cell line were stained with Rat Anti-Mouse LAG-3 PE-conjugated Monoclonal Antibody (Catalog # FAB33281P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse LAG-3 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Mouse splenocytes treated with PMA and Calcium Ionomycin

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LAG-3

LAG-3 (Lymphocyte activation gene-3; also known as CD223 in the human) is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 shares < 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity.  The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3.

Long Name

Lymphocyte-activation Gene 3

Alternate Names

CD223, LAG3

Entrez Gene IDs

3902 (Human); 16768 (Mouse); 297596 (Rat); 102122272 (Cynomolgus Monkey)

Gene Symbol

LAG3

UniProt

Additional LAG-3 Products

Product Documents for Mouse LAG-3 PE-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse LAG-3 PE-conjugated Antibody

For research use only

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