Mouse IGFBP-3 Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB775G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair)
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 138202
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-3
Pro22-Gln291 (Arg250Gln, Gln259Arg, and Arg271Pro)
Accession # P47878
Pro22-Gln291 (Arg250Gln, Gln259Arg, and Arg271Pro)
Accession # P47878
Specificity
Detects mouse IGFBP-3 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human IGFBP‑1, rhIGFBP‑2, rhIGFBP‑3, rhIGFBP‑4, rhIGFBP‑5, rhIGFBP‑6, recombinant mouse (rm) IGFBP‑2, rmIGFBP‑5, or rmIGFB
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for Mouse IGFBP-3 Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
ELISA Capture (Matched Antibody Pair)
Optimal dilution of this antibody should be experimentally determined.
ELISA Detection (Matched Antibody Pair)
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: IGFBP-3
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Mouse IGFBP-3 cDNA encodes a 291 amino acid (aa) residue precursor protein with a putative 27 aa residue signal peptide that is processed to generate the 264 aa residue mature protein with three potential N-linked glycosylation sites. Mouse and human IGFBP-3 share 81% amino acid homology. Mouse IGFBP-3 is expressed in multiple tissues.
Long Name
Insulin-like Growth Factor Binding Protein 3
Alternate Names
IGFBP3
Gene Symbol
IGFBP3
UniProt
Additional IGFBP-3 Products
Product Specific Notices for Mouse IGFBP-3 Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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