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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

E. coli-derived recombinant mouse FoxC2
Ala412-Tyr494
Accession # Q61850

Specificity

Detects mouse FoxC2 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) FoxC2 is observed and less than 3% cross-reactivity with rhFoxF2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse FoxC2 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FoxC2

FOXC2 (Forkhead box protein C2; also BF-3, FKH-14 and MFH-1) is a 64-66 kDa member of the winged helix transcription factor gene family. It is widely expressed, including in endothelium where it induces the appearance of CXCR4 and integrin beta3, two molecules essential to cell migration. High-calorie diets also induce FOXC2 in adipocytes, inducing a brown-fat like phenotype. Mouse FOXC2 is 494 amino acids (aa) in length. It contains a forkhead DNA binding domain (aa 71-148), a short poly-Arg segment (aa 162-166), one His-rich region (aa 386-395) and an Ala/Pro-rich domain (aa 396-415). There are seven potential Ser/Thr phosphorylation sites. Over aa 412-494, mouse FOXC2 shares 99% and 87% aa identity with rat and human FOXC2, respectively.

Long Name

Forkhead Box C2

Alternate Names

Fkh14, LD, MFH1

Entrez Gene IDs

2303 (Human); 14234 (Mouse)

Gene Symbol

FOXC2

UniProt

Additional FoxC2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse FoxC2 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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