Mouse DCC Alexa Fluor® 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF844V
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot, Blockade of Receptor-ligand Interaction
Label
Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse DCC
Phe32-Asn1097
Accession # P70211
Phe32-Asn1097
Accession # P70211
Specificity
Detects mouse DCC in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse DCC Alexa Fluor® 405-conjugated Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: DCC
Deleted in colorectal cancer (DCC) was originally identified as a putative tumor suppressor gene that is lost in more than 70% of colorectal cancers. This gene has also been found to be deleted in several different kinds of cancers. DCC encodes a type I transmembrane glycoprotein that belongs to the immunoglobulin (Ig) superfamily. The extracellular domain is composed of four Ig-like domains and six fibronectin type III repeats. Two forms of the protein (the long and the short isoforms) are produced from the same gene by the use of alternative initiation sites. A third isoform that is produced by alternative splicing is expressed only in the embryo. The extracellular domain of mouse DCC shares 97% and 99% amino acid sequence identity with the human and rat DCC extracellular domains, respectively. In adults, DCC is highly expressed in the brain but is also expressed at very low levels in multiple tissues. In the embryo, high levels of expression are detected in the brain and neural tube. DCC has been shown to be a receptor for the netrins that are important for axon guidance. DCC has also been shown to induce apoptosis in the absence of ligand binding and to block apoptosis when engaged by netrin-1. DCC has been shown to be a caspase substrate. The pro-apoptotic effects of DCC were found to be dependent on the proteolytic cleavage of the unoccupied receptor by caspase. It is likely that DCC functions as a tumor-suppressor gene by inducing apoptosis in cells that are not exposed to netrins.
Long Name
Deleted in Colorectal Cancer
Alternate Names
Colorectal cancer suppressor, CRC18, CRCR1, deleted in colorectal cancer protein, deleted in colorectal carcinoma, IGDCC1colorectal tumor suppressor, Immunoglobulin superfamily DCC subclass member 1, immunoglobulin superfamily, DCC subclass, member 1, netrin receptor DCC, Tumor suppressor protein DCC
Gene Symbol
DCC
UniProt
Additional DCC Products
Product Documents for Mouse DCC Alexa Fluor® 405-conjugated Antibody
Product Specific Notices for Mouse DCC Alexa Fluor® 405-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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