Mouse DC-SIGN/CD209 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8345P

Recombinant Monoclonal Antibody.
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FAB8345P-025
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Conjugate
Catalog #
Unconjugated
MAB8345
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Alexa Fluor 350
FAB8345U
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Alexa Fluor 405
FAB8345V
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Alexa Fluor 488
FAB8345G
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Alexa Fluor 532
FAB8345X
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Alexa Fluor 594
FAB8345T
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Alexa Fluor 647
FAB8345R
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Alexa Fluor 700
FAB8345N
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Alexa Fluor 750
FAB8345S
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Alexa Fluor Plus 405
FAB8345AFP405
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Alexa Fluor Plus 488
FAB8345AFP488
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Alexa Fluor Plus 555
FAB8345AFP555
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Alexa Fluor Plus 594
FAB8345AFP594
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Alexa Fluor Plus 647
FAB8345AFP647
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Alexa Fluor Plus 680
FAB8345AFP680
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Allophycocyanin
FAB8345A
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Allophycocyanin/Cy7
FAB8345APCCY7
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Biotin
FAB8345B
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CoraFluor 1
FAB8345CL1
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DyLight 350
FAB8345D
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DyLight 405
FAB8345E
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DyLight 488
FAB8345K
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DyLight 550
FAB8345L
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DyLight 594
FAB8345M
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DyLight 650
FAB8345W
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DyLight 680
FAB8345Y
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DyLight 755
FAB8345Z
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FITC
FAB8345F
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HRP
FAB8345H
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Janelia Fluor 525
FAB8345JF525
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Janelia Fluor 549
FAB8345I
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Janelia Fluor 646
FAB8345J
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Janelia Fluor 669
FAB8345JF669
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mFluor Violet 450 SE
FAB8345MFV450
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mFluor Violet 500 SE
FAB8345MFV500
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mFluor Violet 610 SE
FAB8345MFV610
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PE/Atto594
FAB8345PEATT594
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PE/Cy5.5
FAB8345PECY55
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PE/Cy7
FAB8345PECY7
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PerCP
FAB8345C
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rat IgG2B Clone # 902404

View all DC-SIGN/CD209 Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with mouse DC‑SIGN/CD209
Met1-Lys238
Accession # Q91ZX1

Specificity

Detects mouse DC‑SIGN/CD209 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse DC-SIGN/CD209 PE-conjugated Antibody

Detection of DC-SIGN/CD209 antibody in Mouse Bone Marrow-derived Dendritic Cells antibody by Flow Cytometry.

Detection of DC‑SIGN/CD209 in Mouse Bone Marrow-derived Dendritic Cells by Flow Cytometry.

Mouse bone marrow-derived dendritic cells were stained with Rat Anti-Mouse DC-SIGN/CD209 PE-conjugated Monoclonal Antibody (Catalog # FAB8345P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of DC-SIGN/CD209 antibody in CHO Chinese Hamster Cell Line Transfected with Mouse DC-SIGN/CD209 antibody by Flow Cytometry.

Detection of DC‑SIGN/CD209 in CHO Chinese Hamster Cell Line Transfected with Mouse DC-SIGN/CD209 by Flow Cytometry.

CHO Chinese hamster ovary cell line transfected with mouse DC-SIGN/CD209 was stained with Rat Anti-Mouse DC-SIGN/CD209 PE-conjugated Monoclonal Antibody (Catalog # FAB8345P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse DC-SIGN/CD209 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse bone marrow-derived dendritic cells and CHO Chinese hamster ovary cell line transfected with mouse DC-SIGN/CD209

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DC-SIGN/CD209

Mouse Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209, also known as CD209 Antigen-like Protein A, is a member of the C-type lectin family (1). Mouse DC-SIGN/CD209 is a 33 kDa, 238 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca2+-dependent carbohydrate-binding lectin domain (2). In addition to the full-length mouse DC-SIGN/CD209, a second, truncated splice variant has been reported. DC-SIGN/CD209 is not well conserved between mouse and human with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2-4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (2, 5, 6).

References

  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Caminschi, I. et al. (2001) Mol. Immunol. 38:365.
  3. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
  5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
  6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1

Alternate Names

CD209, CLEC4L, DC-SIGN1, DCSIGN

Entrez Gene IDs

30835 (Human); 170786 (Mouse); 102121984 (Cynomolgus Monkey)

Gene Symbol

CD209

UniProt

Q91ZX1

Additional DC-SIGN/CD209 Products

Product Documents for Mouse DC-SIGN/CD209 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse DC-SIGN/CD209 PE-conjugated Antibody

For research use only

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