Detects CXCL7/Thymus Chemokine‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant rat CXCL7/Thymus Chemokine-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CXCL7/Thymus Chemokine-1 Antibody

CXCL7/Thymus Chemokine-1 in Mouse Thymus.

CXCL7/Thymus Chemokine-1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CXCL7/Thymus Chemokine-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF793) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Chemotaxis Induced by CXCL7/Thymus Chemo-kine‑1 and Neutralization by Mouse CXCL7/Thymus Chemo-kine‑1 Antibody.

Recombinant Mouse CXCL7/Thymus Chemo-kine-1 (Catalog # 1091-CK) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL7/Thymus Chemo-kine-1 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL7/Thymus Chemo-kine-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF793). The ND₅₀ is typically 0.8-4 µg/mL.

Mouse CXCL7 / Thymus Chemokine-1 ELISA Standard Curve

Recombinant Mouse CXCL7/Thymus Chemokine-1 aa 48-109 (Catalog # 1091-CK) was serially diluted and captured by Rat Anti-Mouse CXCL7/Thymus Chemokine-1 Monoclonal Antibody (Catalog # MAB10911) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL7/Thymus Chemokine-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF793) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse CXCL7/Thymus Chemokine-1 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CXCL7/Thymus Chemokine-1 (Catalog # 793-CK)

Neutralization

Measured by its ability to neutralize CXCL7/Thymus Chemokine-1-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.8-4 µg/mL in the presence of 40 ng/mL Recombinant Mouse CXCL7/Thymus Chemokine-1.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL7/Thymus Chemokine-1

CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Mouse CXCL7 cDNA encodes a 113 amino acid residue precursor protein. Signal P prediction program analysis indicates that this precursor protein contains an amino terminal 39 amino acid residue signal peptide and a 74 amino acid mature chemokine. Mouse CXCL7 shares 72% amino acid sequence identity with rat CXCL7. The recombinant mouse CXCL7 produced by R&D Systems has been shown to chemoattract mouse BaF3 cells transfected with human CXCR2, but not human CXCR1.

Alternate Names

Thymus Chemokine1

Entrez Gene IDs

57349 (Mouse); 246358 (Rat)

Gene Symbol

PPBP

UniProt

AAG36786

Additional CXCL7/Thymus Chemokine-1 Products

Product Documents for Mouse CXCL7/Thymus Chemokine-1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse CXCL7/Thymus Chemokine-1 Antibody

For research use only

Citations
Reviews

Mouse CXCL7/Thymus Chemokine-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF793

Key Product Details

Species Reactivity

Validated:

Cited:

Applications