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Recombinant Mouse CXCL7/Thymus Chemokine-1 (aa 48-109)

Bio-Techne includes R&D Systems | Catalog # 1091-CK

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Carrier Free
1091-CK-025/CF

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With Carrier
1091-CK-025

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived mouse CXCL7/Thymus Chemokine-1 protein
Ile48-Ile109

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

N-terminal Sequence Analysis

Ile48

Predicted Molecular Mass

6.8 kDa

Activity

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CXCR2.
The ED50 for this effect is 1-5 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1091-CK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1091-CK/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL7/Thymus Chemokine-1

CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Mouse CXCL7 cDNA encodes a 113 amino acid residue precursor protein that shares 72% amino acid sequence identity with rat CXCL7 (accession number AAK30166). The recombinant mouse CXCL7 produced by R&D Systems has been shown to chemoattract human CXCR2 transfected mouse BaF/3 cells, but not human CXCR1 transfected mouse BaF3 cells. Two isoforms of mouse CXCL7 have been produced at R&D Systems. The Ile48 - Ile109 mouse CXCL7 isoform (R&D Systems, Catalog # 1091-CK) has been shown to be 200-fold more potent than the Lys40 - Tyr113 isoform (R&D Systems, Catalog # 763-CK) as a chemoattractant for mouse BaF/3 cells transfected with human CXCR2.

Alternate Names

Thymus Chemokine1

Entrez Gene IDs

57349 (Mouse); 246358 (Rat)

Gene Symbol

PPBP

UniProt

Additional CXCL7/Thymus Chemokine-1 Products

Product Documents for Recombinant Mouse CXCL7/Thymus Chemokine-1 (aa 48-109)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse CXCL7/Thymus Chemokine-1 (aa 48-109)

For research use only

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