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Mouse CD4 PE‑conjugated Antibody
Catalog # FAB554P | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # GK1.5
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Product Summary for Mouse CD4 PE‑conjugated Antibody
Immunogen
Mouse CTL clone V4
Specificity
Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse CD4 PE‑conjugated Antibody
Detection of CD4 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) or (B) Rat IgG2BPhycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse CD4 PE‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD4
Product Documents for Mouse CD4 PE‑conjugated Antibody
Product Specific Notices for Mouse CD4 PE‑conjugated Antibody
For research use only
Citations for Mouse CD4 PE‑conjugated Antibody (4)
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Citations are publications that use Bio-Techne products. Selected citations for Mouse CD4 PE‑conjugated Antibody include:
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Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryJ Knapp et al. (2022-12-12), HSPB5 suppresses renal inflammation and protects lupus-prone NZB/W F1 mice from severe renal damage Arthritis Research & Therapy, 2022-124(1):267.
PMID: 36510250 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryAzukizawa H et al. (2011-04-14), Steady state migratory RelB+ langerin+ dermal dendritic cells mediate peripheral induction of antigen-specific CD4+ CD25+ Foxp3+ regulatory T cells. Eur. J. Immunol., 2011-041(5):1420-34.
PMID: 21469094 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryNotley CA et al. (2011-03-07), Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells. J. Immunol., 2011-0186(8):4967-72.
PMID: 21383247 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometrySiegemund S et al. (2009-03-18), Differential IL-23 requirement for IL-22 and IL-17A production during innate immunity against Salmonella enterica serovar Enteritidis. Int. Immunol., 2009-021(5):555-65.
PMID: 19297659
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Customer Reviews for Mouse CD4 PE‑conjugated Antibody (1)
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Application: Flow CytometrySample Tested: Adult small intestineSpecies: MouseVerified Customer | Posted 01/25/2018
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FAQs for Mouse CD4 PE‑conjugated Antibody
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Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
A: Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
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