Mouse CD11b/Integrin alpha M Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11242
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Multiplex Immunofluorescence, Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1250D
Product Specifications
Immunogen
MouseCD11b/Integrin alpha M synthetic peptide
Accession # P11215
Accession # P11215
Specificity
Detects mouse CD11b/Integrin alpha M in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Mouse CD11b/Integrin alpha M Antibody
Detection of CD11b in Mouse Spleen via seqIF™ staining on COMET™
CD11b was detected in immersion fixed paraffin-embedded sections of mouse Spleen using Rabbit Anti-Mouse CD11b, Monoclonal Antibody (Catalog #MAB11242) at 3ug/mL dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.
Detection of Mouse CD11b/Integrin alpha M by Western Blot.
Western blot shows lysates of mouse brain. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB11242) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for CD11b/Integrin alpha M at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Mouse and Rat CD11b/Integrin alpha M by Simple WesternTM.
Simple Western lane view shows lysates of mouse brain and rat brain, loaded at 0.2 mg/mL. A specific band was detected for CD11b/Integrin alpha M at approximately 180-200 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB11242) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse CD11b/Integrin alpha M Antibody
Application
Recommended Usage
Multiplex Immunofluorescence
3 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse spleen
Sample: Immersion fixed paraffin-embedded sections of mouse spleen
Simple Western
5 µg/mL
Sample: Mouse brain and rat brain
Sample: Mouse brain and rat brain
Western Blot
0.5 µg/mL
Sample: Mouse brain
Sample: Mouse brain
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD11b/Integrin alpha M
References
- Springer, T.A. et al. (1978) Eur. J. Immunol. 8:539.
- Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.
- Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
Alternate Names
CD11b, Integrin alpha M, ITGAM
Gene Symbol
ITGAM
UniProt
Additional CD11b/Integrin alpha M Products
Product Documents for Mouse CD11b/Integrin alpha M Antibody
Product Specific Notices for Mouse CD11b/Integrin alpha M Antibody
For research use only
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