Detects mouse CCL12/MCP-5 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) MCP‑3 is observed and 5% cross-reactivity with recombinant mouse (rm) JE, rmMARC, rhMCP‑1, and rhMCP‑2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL12/MCP-5 Antibody

Chemotaxis Induced by CCL12/MCP-5 and Neutralization by Mouse CCL12/MCP-5 Antibody.

Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/MCP-5 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL12/MCP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF428). The ND₅₀ is typically 0.8-4.0 µg/mL.

Applications for Mouse CCL12/MCP-5 Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5)

Neutralization

Measured by its ability to neutralize CCL12/MCP-5-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR2A. The Neutralization Dose (ND₅₀) is typically 0.8-4.0 µg/mL in the presence of 0.2 µg/mL Recombinant Mouse CCL12/MCP-5.

Reviewed Applications

Read 1 review rated 4 using AF428 in the following applications:

Block/Neutralize (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL12/MCP-5

CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid (aa) sequence identity in the mature protein). Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.

References

Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.

Alternate Names

MCP-5, MCP5

Entrez Gene IDs

20293 (Mouse)

Gene Symbol

CCL12

UniProt

Q62401

Additional CCL12/MCP-5 Products

Product Documents for Mouse CCL12/MCP-5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CCL12/MCP-5 Antibody

For research use only

Citations
Reviews

Mouse CCL12/MCP-5 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF428

Key Product Details

Species Reactivity

Validated:

Cited:

Applications