Mkl1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88498

Novus Biologicals, part of Bio-Techne
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NBP1-88498-25ul
NBP1-88498
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Mkl1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EQEKRAQQPAPAPAPLGTPVKQENSFSSCQLSQQPLGPAHPFNPSLAAPATNHIDPCAVAPGPPSVVVKQEALQPEPEPVPAPQLLLGPQGPSLIKGVAPPTLITDSTGTHLVLTVTNKNADSPG

Reactivity Notes

Mouse reactivity reported in the scientific literature (PMID: 26165921)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Mkl1 Antibody - BSA Free (NBP1-88498) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Mkl1 Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Mkl1 Antibody - BSA Free

Mkl1 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: Mkl1 Antibody - BSA Free [NBP1-88498]

Immunocytochemistry/ Immunofluorescence: Mkl1 Antibody - BSA Free [NBP1-88498]

Staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498]

Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498]

Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human testis shows positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498]

Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498]

Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human liver shows very weak positivity in hepatocytes.
View 3 more images

Applications for Mkl1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mkl1

MKL1 (megakaryoblastic leukemia 1 protein) was identified as part of a t(1;22) translocation with RBM15 associated with acute megakaryoblastic leukemia. It is a transcriptional coactivator of serum response factor (SRF) that promotes muscle differentiation and also plays a role in promoting maturation of megakaryocytes.

Long Name

Myocardin-related transcription factor A

Alternate Names

KIAA1438, MAL, MRTF-A, MRTFA

Entrez Gene IDs

57591 (Human)

Gene Symbol

MRTFA

UniProt

Q969V6 (Human)

Additional Mkl1 Products

Product Documents for Mkl1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Mkl1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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