MKK3/MKK6 Antibody (8R1J5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15946
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8R1J5 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 248-347 of human MKK3/MKK6 (P46734). KSDVWSLGITMIEMAILRFPYESWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTAQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit MKK3/MKK6 Antibody (8R1J5) (NBP3-15946) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MKK3/MKK6 Antibody (8R1J5)
![Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946]](https://resources.bio-techne.com/images/products/MKK3-MKK6-Antibody-8R1J5-Western-Blot-NBP3-15946-img0001.jpg "Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946]")
Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946]
Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946] - Western blot analysis of extracts of various cell lines, using MKK3/MKK6 Rabbit mAb (NBP3-15946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946]](https://resources.bio-techne.com/images/products/MKK3-MKK6-Antibody-8R1J5-Western-Blot-NBP3-15946-img0002.jpg "Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946]")
Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946]
Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946] - Western blot analysis of extracts of various cell lines, using MKK3/MKK6 Rabbit mAb (NBP3-15946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Applications for MKK3/MKK6 Antibody (8R1J5)
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MKK3/MKK6
Mitogen-activated protein kinase kinase 3 (MKK3), also known as MEK3, and the closely related MKK6 are activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation by several upstream MAPK kinase kinases, including ASK1 and TAK1. Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr.
Long Name
Mitogen-activated Protein Kinase Kinase 3
Alternate Names
Dual specificity mitogen-activated protein kinase kinase 6;MAP2K6;MAPKK6;MEK6;MKK6;PRKMK6;SAPKK3;SAPKK-3
Gene Symbol
MAP2K6
Additional MKK3/MKK6 Products
Product Documents for MKK3/MKK6 Antibody (8R1J5)
Product Specific Notices for MKK3/MKK6 Antibody (8R1J5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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