Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Menin Antibodies »

Product Specifications

Immunogen

A synthetic peptide, which represented a portion of human Multiple Endocrine Neoplasia 1 encoded within exon 11 (MEN1 LocusLink ID 4221).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Menin Antibody - BSA Free

Western Blot: Menin Antibody [NB100-399]

Western Blot: Menin Antibody [NB100-399]

Western Blot: Menin Antibody [NB100-399] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody (lot was used at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: Menin Antibody [NB100-399]

Western Blot: Menin Antibody [NB100-399]

Western Blot: Menin Antibody [NB100-399] - A. Whole cell lysate (40 mcg - E; 10 mcg - T) from 293T cells untreated (E) or transfected with a Menin expression construct (T). B. Whole cell lysate from one 10 cm plate of untreated 293T cells. A. Affinity purified rabbit anti-Menin antibody NB100-399 used at the indicated concentrations for WB. B. Affinity purified rabbit anti-Menin antibodies NB100-215 (5 mcg/plate), NB100-398(10 mcg/plate). Immunoprecipitated Menin was detected using NB100-215 at 0.1 mcg/ml.
Western Blot: Menin Antibody [NB100-399]

Western Blot: Menin Antibody [NB100-399]

Western Blot: Menin Antibody [NB100-399] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Menin Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg of lysate

Western Blot

1:500-1:5000
Application Notes
This antibody may be used for Western Blot, where a doublet band should be seen at ~72 kDa. Suggested working dilutions: * Western Blot - 1:500-1:5,000 Immunoprecipitation - 2 to 10 ug/mg lysate Immunofluorescence - ND *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Menin

Multiple Endocrine Neoplasia is an autosomal dominant disorder characterized by a high frequency of peptic ulcer disease and primary endocrine abnormalities involving the pituitary, parathyroid, and pancreas.

Long Name

Multiple Endocrine Neoplasia I

Alternate Names

MEAI, MEN1

Entrez Gene IDs

4221 (Human)

Gene Symbol

MEN1

Additional Menin Products

Product Documents for Menin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Menin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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