MED8 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89014
![MED8 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: MED8 Antibody - BSA Free [NBP1-89014]](https://resources.bio-techne.com/images/products/nbp1-89014_rabbit-polyclonal-med8-antibody-256202599320.jpg "Chromatin Immunoprecipitation-exo-Seq: MED8 Antibody - BSA Free [NBP1-89014]")
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (94%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LLEKISKEERESESGGLRPNKQTFNPTDTNALVAAVAFGKGLSNWRPSGSSGPGQAGQPGAGTILAGTSGLQ
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit MED8 Antibody - BSA Free (NBP1-89014) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MED8 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: MED8 Antibody [NBP1-89014]](https://resources.bio-techne.com/images/products/MED8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89014-img0008.jpg "Immunocytochemistry/ Immunofluorescence: MED8 Antibody [NBP1-89014]")
Immunocytochemistry/ Immunofluorescence: MED8 Antibody [NBP1-89014]
Immunocytochemistry/Immunofluorescence: MED8 Antibody [NBP1-89014] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.![Immunohistochemistry-Paraffin: MED8 Antibody [NBP1-89014]](https://resources.bio-techne.com/images/products/MED8-Antibody-Immunohistochemistry-Paraffin-NBP1-89014-img0007.jpg "Immunohistochemistry-Paraffin: MED8 Antibody [NBP1-89014]")
Immunohistochemistry-Paraffin: MED8 Antibody [NBP1-89014]
Immunohistochemistry-Paraffin: MED8 Antibody [NBP1-89014] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.![Western Blot: MED8 Antibody [NBP1-89014]](https://resources.bio-techne.com/images/products/MED8-Antibody-Western-Blot-NBP1-89014-img0006.jpg "Western Blot: MED8 Antibody [NBP1-89014]")
Western Blot: MED8 Antibody [NBP1-89014]
Western Blot: MED8 Antibody [NBP1-89014] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG spApplications for MED8 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MED8
Alternate Names
ARC32subunit 8 homolog, mediator complex subunit 8mediator of RNA polymerase II transcription, subunit 8 homolog (S. cerevisiae)
Gene Symbol
MED8
Additional MED8 Products
Product Documents for MED8 Antibody - BSA Free
Product Specific Notices for MED8 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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