Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Western Blot (Negative)

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all MED23 Antibodies »

Product Specifications

Immunogen

A synthetic peptide that maps to a region between residues 1 and 50 of human Cofactor Required for Sp1 transcriptional activation, subunit 3 using the numbering given in Swiss-Prot entry Q9ULK4 (GeneID 9439).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25892301).

Specificity

NB200-337 is specific for human CRSP 3 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit MED23 Antibody - BSA Free (NB200-337) is a polyclonal antibody validated for use in IP and ChIP. Anti-MED23 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MED23 Antibody - BSA Free

Immunoprecipitation: MED23 Antibody [NB200-337]

Immunoprecipitation: MED23 Antibody [NB200-337]

Immunoprecipitation: MED23 Antibody [NB200-337] - Nuclear extract (5 mg) from HeLa cells. Antibody used at 4 ug/5 mg extract.

Applications for MED23 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunoprecipitation

1-4 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Western Blot: Not Recommended, use NB 200-339 for WB. *In some cases, the antibody may be diluted further than indicated. Use in chromatin immunoprecipitation reported in scientific literature (PMID 25892301)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MED23

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Alternate Names

130 kDa transcriptional co-activator, 133 kDa transcriptional co-activator, CRSP130, DRIP130DKFZp434H0117, mediator complex subunit 23CRSP133, Protein sur-2 homolog, subunit 3, 130kDa, Transcriptional coactivator CRSP130, Vitamin D3 receptor-interacting protein complex 130 kDa component

Entrez Gene IDs

9439 (Human)

Gene Symbol

MED23

Additional MED23 Products

Product Documents for MED23 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MED23 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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