MDMX Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-556
![Western Blot: MDMX Antibody [NB100-556]](https://resources.bio-techne.com/images/products/MDMX-Antibody-Western-Blot-NB100-556-img0008.jpg "Western Blot: MDMX Antibody [NB100-556]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 125 and 175 of human ortholog of mouse double minute 4 using the numbering given in Swiss-Prot entry O15151 (GeneID 4194).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit MDMX Antibody - BSA Free (NB100-556) is a polyclonal antibody validated for use in WB and IP. Anti-MDMX Antibody: Cited in 33 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MDMX Antibody - BSA Free
Western Blot: MDMX Antibody [NB100-556]
Western Blot: MDMX Antibody [NB100-556] - Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.![Western Blot: MDMX Antibody [NB100-556]](https://resources.bio-techne.com/images/products/MDMX-Antibody-Western-Blot-NB100-556-img0006.jpg "Western Blot: MDMX Antibody [NB100-556]")
Western Blot: MDMX Antibody [NB100-556]
Western Blot: MDMX Antibody [NB100-556] - Analysis using the Biotin conjugate of NB100-556. Detection of Jurkat (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: MDMX Antibody [NB100-556]](https://resources.bio-techne.com/images/products/MDMX-Antibody-Immunoprecipitation-NB100-556-img0009.jpg "Immunoprecipitation: MDMX Antibody [NB100-556]")
Immunoprecipitation: MDMX Antibody [NB100-556]
Immunoprecipitation: MDMX Antibody [NB100-556] - Detection of human HdmX/MDM4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 (lot NB100-556-4) used for IP at 6 ug per reaction. HdmX/MDM4 was also immunoprecipitated by a previous lot of this antibody (lot NB100-556-3) and another rabbit anti-HdmX/MDM4 antibody. For blotting immunoprecipitated HdmX/MDM4, NB100-556 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for MDMX Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 -10 ug/mg lysate
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MDM4/MDMX
Long Name
Double Minute 4 Protein
Alternate Names
MDM4 Regulator of P53, MDMX, MRP1
Entrez Gene IDs
4194 (Human)
Gene Symbol
MDM4
UniProt
Additional MDM4/MDMX Products
Product Documents for MDMX Antibody - BSA Free
Product Specific Notices for MDMX Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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