MDH1 Antibody (JE35-03)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32582
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE35-03 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide. (Uniprot: P40925)
Localization
Cytoplasm, cytosol.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Knockout (KO) Validated Rabbit MDH1 Antibody (JE35-03) (NBP3-32582) is a recombinant monoclonal antibody validated for use in WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MDH1 Antibody (JE35-03)
Western Blot Shows MDH1 Specificity Using Knockout Cell Line.
Western blot shows lysates of HAP1 parental cell line and MDH1 knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with MDH1 Antibody (JE35-03) (Catalog # NBP3-32582) followed by HRP-conjugated secondary antibody. A specific band was detected for MDH1 at approximately 36.4 kDa (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
Detection of MDH1 by Immunoprecipitation.
HAP1 cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of MDH1 Antibody (JE35-03) (Catalog # NBP3-32582) pre-coupled to Dynabeads Protein A. Immunoprecipitated MDH1 was detected in Western Blot with a rabbit MDH1 antibody used at 1/10000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
MDH1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.
HAP1 parental cell line WT and MDH1 HAP1 KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with MDH1 Antibody (JE35-03) (Catalog # NBP3-32582) followed by incubation with an Alexa-fluor 555 conjugated secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Applications for MDH1 Antibody (JE35-03)
Application
Recommended Usage
Flow Cytometry
1:1000
Immunocytochemistry/ Immunofluorescence
1:100
Immunoprecipitation
Validated for Immunoprecipitation from YCharOS Inc. (YCharOS.com)
Knockout Validated
Knockout Validated from YCharOS Inc. (YCharOS.com)
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MDH1
Long Name
malate dehydrogenase 1
Alternate Names
DEE88, EIEE88, HEL-S-32, KAR, MDH-s, MDHA, MGC:1375, MOR2
Gene Symbol
MDH1
Additional MDH1 Products
Product Documents for MDH1 Antibody (JE35-03)
Product Specific Notices for MDH1 Antibody (JE35-03)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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