MCM6 Antibody (23GB2005)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50969

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NBP3-50969-100ul
NBP3-50969-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2005

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MCM6 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human MCM6 (Uniprot: Q14566)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM6 Antibody (23GB2005)

MCM6 Antibody (23GB2005)

Immunocytochemistry/ Immunofluorescence: MCM6 Antibody (23GB2005)[NBP3-50969] -

Immunocytochemical staining of C2C12 cells with MCM6 antibody (1:1,000). Nuclei were stained blue with DAPI; MCM6 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
MCM6 Antibody (23GB2005)

Flow Cytometry: MCM6 Antibody (23GB2005)[NBP3-50969] -

Validation of MCM6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with MCM6 antibody (1:2,000) and analyzed using BD flow cytometer.
MCM6 Antibody (23GB2005)

Flow Cytometry: MCM6 Antibody (23GB2005)[NBP3-50969] -

Flow cytometric analysis of MCM6expression in C2C12 cells using MCM6 antibody (1:2,000). Green, isotype control; red, MCM6.
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Applications for MCM6 Antibody (23GB2005)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MCM6

MCM6 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.

Alternate Names

DNA replication licensing factor MCM6, EC 3.6.4.12, MCG40308, MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe), MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S.cerevisiae), minichromosome maintenance complex component 6, minichromosome maintenance deficient (mis5, S. pombe) 6, minichromosome maintenance deficient 6 homolog, minichromosome maintenance deficient 6 homolog (S. cerevisiae), Mis5, MIS5 homolog, p105MCM

Gene Symbol

MCM6

Additional MCM6 Products

Product Documents for MCM6 Antibody (23GB2005)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MCM6 Antibody (23GB2005)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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