MCAM/CD146 Antibody (P1H12) [mFluor Violet 500 SE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43346MFV500

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Product Datasheet / COA / SDS

Clone P1H12 was used by HLDA to establish CD designation.

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Conjugate

Catalog #

Alexa Fluor 350

NBP1-43346AF350

Alexa Fluor 405

NBP1-43346AF405

Alexa Fluor 488

NBP1-43346AF488

Alexa Fluor 532

NBP1-43346AF532

Alexa Fluor 594

NBP1-43346AF594

Alexa Fluor 647

NBP1-43346AF647

Alexa Fluor 700

NBP1-43346AF700

Alexa Fluor 750

NBP1-43346AF750

Alexa Fluor Plus 405

NBP1-43346AFP405

Alexa Fluor Plus 488

NBP1-43346AFP488

Alexa Fluor Plus 555

NBP1-43346AFP555

Alexa Fluor Plus 594

NBP1-43346AFP594

Alexa Fluor Plus 647

NBP1-43346AFP647

Alexa Fluor Plus 680

NBP1-43346AFP680

Allophycocyanin

NBP1-43346APC

Allophycocyanin/Cy7

NBP1-43346APCCY7

Biotin

NBP1-43346B

CoraFluor 1

NBP1-43346CL1

DyLight 350

NBP1-43346UV

DyLight 405

NBP1-43346V

DyLight 488

NBP1-43346G

DyLight 550

NBP1-43346R

DyLight 594

NBP1-43346DL594

DyLight 650

NBP1-43346C

DyLight 680

NBP1-43346FR

DyLight 755

NBP1-43346IR

FITC

NBP1-43346F

HRP

NBP1-43346H

Janelia Fluor 525

NBP1-43346JF525

Janelia Fluor 549

NBP1-43346JF549

Janelia Fluor 646

NBP1-43346JF646

Janelia Fluor 669

NBP1-43346JF669

mFluor Violet 450 SE

NBP1-43346MFV450

mFluor Violet 610 SE

NBP1-43346MFV610

PE

NBP1-43346PE

PE/Atto594

NBP1-43346PEATT594

PE/Cy5.5

NBP1-43346PECY55

PE/Cy7

NBP1-43346PECY7

PerCP

NBP1-43346PCP

Forumulation

Catalog #

BSA Free

NBP1-43346

Product Specifications
Scientific Data
Applications
Spectra Viewer
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Canine, Rabbit, Rat (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation

Label

mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # P1H12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MCAM/CD146 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD146.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Scientific Data Images for MCAM/CD146 Antibody (P1H12) [mFluor Violet 500 SE]

MCAM/CD146 Antibody (P1H12) [mFluor Violet 500 SE] -

MCAM/CD146 Antibody (P1H12) [mFluor Violet 500 SE] - Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an emission maximum of 501 nm.

Applications for MCAM/CD146 Antibody (P1H12) [mFluor Violet 500 SE]

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Recommended applications are based on validated applications from the unconjugated base product NBP1-43346. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: MCAM/CD146

CD146 belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety. Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Over expression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Gene Symbol

MCAM

Additional MCAM/CD146 Products

Product Documents for MCAM/CD146 Antibody (P1H12) [mFluor Violet 500 SE]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for MCAM/CD146 Antibody (P1H12) [mFluor Violet 500 SE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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