MARCH3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81260
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (96%), Rat (94%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FRYHCRLYNEWRRTNQRVILLIPKSVNVPSNQPSLLGLHSVKRNSKETVV
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit MARCH3 Antibody - BSA Free (NBP1-81260) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MARCH3 Antibody - BSA Free
![Western Blot: MARCH3 Antibody [NBP1-81260]](https://resources.bio-techne.com/images/products/MARCH3-Antibody-Western-Blot-NBP1-81260-img0009.jpg "Western Blot: MARCH3 Antibody [NBP1-81260]")
Western Blot: MARCH3 Antibody [NBP1-81260]
Western Blot: MARCH3 Antibody [NBP1-81260] - Analysis in control (vector only transfected HEK293T lysate) and LY405925 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: MARCH3 Antibody [NBP1-81260]](https://resources.bio-techne.com/images/products/MARCH3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81260-img0008.jpg "Immunocytochemistry/ Immunofluorescence: MARCH3 Antibody [NBP1-81260]")
Immunocytochemistry/ Immunofluorescence: MARCH3 Antibody [NBP1-81260]
Immunocytochemistry/Immunofluorescence: MARCH3 Antibody [NBP1-81260] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: MARCH3 Antibody [NBP1-81260]](https://resources.bio-techne.com/images/products/MARCH3-Antibody-Immunohistochemistry-Paraffin-NBP1-81260-img0004.jpg "Immunohistochemistry-Paraffin: MARCH3 Antibody [NBP1-81260]")
Immunohistochemistry-Paraffin: MARCH3 Antibody [NBP1-81260]
Immunohistochemistry-Paraffin: MARCH3 Antibody [NBP1-81260] - Staining of human kidney shows strong cytoplasmic positivity in a subset of renal tubules.Applications for MARCH3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MARCH3
Alternate Names
EC 6.3.2, EC 6.3.2.-, MARCH-IIIFLJ20096, membrane-associated ring finger (C3HC4) 3, Membrane-associated RING finger protein 3, Membrane-associated RING-CH protein III, MGC48332, RING finger protein 173, RNF173E3 ubiquitin-protein ligase MARCH3
Entrez Gene IDs
115123 (Human)
Gene Symbol
MARCH3
UniProt
Additional MARCH3 Products
Product Documents for MARCH3 Antibody - BSA Free
Product Specific Notices for MARCH3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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