LSD1 Antibody (1B2E5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1762

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Simple Western, Chromatin Immunoprecipitation (ChIP), Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, ELISA, Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B2E5

Format

BSA Free

Concentration

1.0 mg/ml

View all Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human LSD1 (between amino acids 400-600) expressed in E. coli. [UniProt# O60341]

Reactivity Notes

Human, mouse and monkey.

Localization

Nucleus

Marker

Nucleus Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse LSD1 Antibody (1B2E5) - BSA Free (NB100-1762) is a monoclonal antibody validated for use in IHC, WB, ELISA, Simple Western and ChIP. Anti-LSD1 Antibody: Cited in 12 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LSD1 Antibody (1B2E5) - BSA Free

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

LSD1-Antibody-1B2E5-Western-Blot-NB100-1762-img0007.jpg
Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762]

Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762]

LSD1-Antibody-1B2E5-Immunohistochemistry-Paraffin-NB100-1762-img0006.jpg
Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.
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Applications for LSD1 Antibody (1B2E5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Simple Western

1:500

Western Blot

1:500-1:2000
Application Notes
This LSD1 (1B2E5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:500, apparent MW was 120 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 1A/KDM1A/LSD1

The amine oxidase domain 2 AOF2 gene encodes a nuclear protein (LSD1,~95 kDa) containing a Swirm domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. LSD1 is a chromatin-modifying enzyme, which serves as a docking module for the stabilization of the associated corepressor complex(es) on chromatin.

Alternate Names

AOF2, BHC110, LSD1, Lysine (K)specific Demethylase 1A

Entrez Gene IDs

23028 (Human)

Gene Symbol

KDM1A

UniProt

O60341 (Human)

Additional Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Products

Product Documents for LSD1 Antibody (1B2E5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LSD1 Antibody (1B2E5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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