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LRRFIP1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84665

Novus Biologicals, part of Bio-Techne
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NBP1-84665
NBP1-84665-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KTEVPGSPAGTEGNCQEATGPSTVDTQNEPLDMKEPDEEKSDQQGEALDSSQKKTKNKKKKNKKKKSPVPVETLKDVKKELTYQNTDLSEIKEEEQVKSTDRKSAVEAQNEVTENPKQKIAAESSENVDCPENPKIKLDGKLDQEGDDV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit LRRFIP1 Antibody - BSA Free (NBP1-84665) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LRRFIP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: LRRFIP1 Antibody [NBP1-84665]

Immunocytochemistry/ Immunofluorescence: LRRFIP1 Antibody [NBP1-84665]

Immunocytochemistry/Immunofluorescence: LRRFIP1 Antibody [NBP1-84665] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: LRRFIP1 Antibody [NBP1-84665]

Immunohistochemistry-Paraffin: LRRFIP1 Antibody [NBP1-84665]

Immunohistochemistry-Paraffin: LRRFIP1 Antibody [NBP1-84665] - Staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.
LRRFIP1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: LRRFIP1 Antibody - BSA Free [NBP1-84665]

Chromatin Immunoprecipitation-exo-Seq: LRRFIP1 Antibody - BSA Free [NBP1-84665]

ChIP-Exo-Seq composite graph for Anti-LRRFIP1 (NBP1-84665) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for LRRFIP1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LRRFIP1

Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA

Alternate Names

FLAP-1, FLIIAP1, GC-binding factor 2, GCF2, GCF-2, HUFI-1, leucine rich repeat (in FLII) interacting protein 1, leucine-rich repeat flightless-interacting protein 1, LRR FLII-interacting protein 1, MGC10947, MGC119738, MGC119739, NEDD8-conjugating enzyme, TRIPTAR RNA-interacting protein

Entrez Gene IDs

9208 (Human)

Gene Symbol

LRRFIP1

UniProt

Additional LRRFIP1 Products

Product Documents for LRRFIP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LRRFIP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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