LIN-28A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49537

Novus Biologicals, part of Bio-Techne
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NBP1-49537
NBP1-49537SS
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all LIN-28A Antibodies »

Product Specifications

Immunogen

Partial recombinant human Lin28 protein expressed in E. coli. [Swiss-Prot# Q9H9Z2]

Localization

Cytoplasm. Nucleus - nucleolus. Notes: Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes. Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.

Marker

Undifferentiated human embryonic stem cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LIN-28A Antibody - BSA Free

Western Blot: LIN-28A Antibody [NBP1-49537]

Western Blot: LIN-28A Antibody [NBP1-49537]

LIN-28A-Antibody-Western-Blot-NBP1-49537-img0013.jpg
Immunocytochemistry/ Immunofluorescence: LIN-28A Antibody [NBP1-49537]

Immunocytochemistry/ Immunofluorescence: LIN-28A Antibody [NBP1-49537]

LIN-28A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49537-img0015.jpg
Immunohistochemistry: LIN-28A Antibody [NBP1-49537]

Immunohistochemistry: LIN-28A Antibody [NBP1-49537]

Immunohistochemistry: LIN-28A Antibody [NBP1-49537] - Analysis of LIN28 in E17.5 mouse embryo.
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Applications for LIN-28A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10-15 ug/ml

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000
Application Notes
This Lin28 antibody is useful for IHC-P, ICC/IF and Western blot.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LIN-28A

Lin28 acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Lin28 is a marker of undifferentiated human embryonic stem cells and has been used to enhance the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. Lin28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells.

Long Name

RNA-binding Protein LIN-28

Alternate Names

CSDD1, LIN28, LIN28A, Tex17, ZCCHC1

Entrez Gene IDs

79727 (Human); 83557 (Mouse)

Gene Symbol

LIN28A

UniProt

Q9H9Z2, Q8K3Y3 (Mouse)

Additional LIN-28A Products

Product Documents for LIN-28A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LIN-28A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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