Lefty1/2 Antibody (JE38-88)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33142
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE38-88 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide. (Uniprot: O75610)
Localization
Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Lefty1/2 Antibody (JE38-88)
![Lefty1/2 Antibody (JE38-88) Western Blot: Lefty1/2 Antibody (JE38-88) [NBP3-33142]](https://resources.bio-techne.com/images/products/nbp3-33142_-western-blot-639204597488691591.jpg "Western Blot: Lefty1/2 Antibody (JE38-88) [NBP3-33142]")
Western Blot: Lefty1/2 Antibody (JE38-88) [NBP3-33142]
Western blot analysis on different lysates with NBP3-33142 at 1/1,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: SiHa cell lysate (15 µg/Lane) Lane 3: NCCIT cell lysate (15 µg/Lane) Lane 4: HeLa cell lysate (15 µg/Lane) Lane 5: ES-D3 cell lysate (15 µg/Lane) Lane 6: F9 cell lysate (15 µg/Lane) Lane 7: ES-W4 cell lysate (15 µg/Lane) Lane 8: Mouse heart tissue lysate (30 µg/Lane) Lane 9: Mouse lung tissue lysate (30 µg/Lane) Lane 10: Mouse embryo tissue lysate (30 µg/Lane) Lane 11: Rat heart tissue lysate (30 µg/Lane) Lane 12: Rat lung tissue lysate (30 µg/Lane) Lane 13: Rat embryo tissue lysate (30 µg/Lane) Predicted band size: 41 kDa Observed band size: 28-41 kDa Exposure time: 1 minute 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33142 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.Applications for Lefty1/2 Antibody (JE38-88)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Lefty1/2
Alternate Names
Beta-4 (Endometrial Bleeding-Associated Factor; LEFTY A), EBAF, endometrial bleeding associated factor (left-right determination, Endometrial Bleeding Associated Factor (Left-Right Determination, Factor A;, Endometrial Bleeding-Associated Factor, LEFTA, Left-Right Determination Factor 2, Left-Right Determination Factor A, LEFTY2, LEFTYA, Protein Lefty-2, Protein Lefty-A, TGFB4, TGF-beta-4, Transforming Growth Factor, Transforming Growth Factor Beta-4
Gene Symbol
LEFTY2
Additional Lefty1/2 Products
Product Documents for Lefty1/2 Antibody (JE38-88)
Product Specific Notices for Lefty1/2 Antibody (JE38-88)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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