Lefty-2 Antibody [PE]
Novus Biologicals, part of Bio-Techne | Catalog # AF7648P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunocytochemistry, Western Blot
Label
PE (Excitation = 488 nm, Emission = 575 nm)
Antibody Source
Polyclonal Sheep IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Lefty-2
Phe78-Leu368
Accession # P57785
Phe78-Leu368
Accession # P57785
Specificity
Detects mouse Lefty-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 65% cross-reactivity with recombinant mouse Lefty-1 is observed, and approximately 20% cross-reactivity with recombinant human Lefty-2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Lefty-2 Antibody [PE]
Application
Recommended Usage
Immunocytochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Lefty-2
Lefty proteins are novel TGF-beta ligands that function as antagonists of Nodal signaling. The expression of Lefty proteins on the left side of the developing mouse embryo earned this protein family its name. Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A and Lefty-B). By amino acid sequence, human Lefty-A and Lefty-B are more similar to each other (96%) than to either Lefty-1 or Lefty-2 in the mouse (81-82%). The Leftys contains some of the features of the cysteine-knot structure conserved among TGF-beta-related proteins, but also some structural differences. Specifically, they lack the alpha-helical segment important for ligand dimerization as well as the cysteine residue involved in stabilization of dimers.
Alternate Names
Ebaf, TGF-beta 4, TGFB4
Gene Symbol
LEFTY2
Additional Lefty-2 Products
Product Documents for Lefty-2 Antibody [PE]
Product Specific Notices for Lefty-2 Antibody [PE]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...