LAMC2 Antibody (CL2980) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44421

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2980

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all LAMC2 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q13753, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse LAMC2 Antibody (CL2980) - Azide and BSA Free (NBP2-42388) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LAMC2 Antibody (CL2980) - Azide and BSA Free

LAMC2 Antibody (CL2980) - Azide and BSA Free Immunohistochemistry-Paraffin: LAMC2 Antibody [NBP3-44421]

Immunohistochemistry-Paraffin: LAMC2 Antibody [NBP3-44421]

Analysis in human fallopian tube and liver tissues using NBP3-44421 antibody. Corresponding LAMC2 RNA-seq data are presented for the same tissues.
LAMC2 Antibody (CL2980) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: LAMC2 Antibody [NBP3-44421]

Immunocytochemistry/ Immunofluorescence: LAMC2 Antibody [NBP3-44421]

Staining of A-431 cells using the Anti-LAMC2 monoclonal antibody) .
LAMC2 Antibody (CL2980) - Azide and BSA Free Immunohistochemistry-Paraffin: LAMC2 Antibody [NBP3-44421]

Immunohistochemistry-Paraffin: LAMC2 Antibody [NBP3-44421]

Staining of human fallopian tube shows moderate positivity in basement membrane of glandular cells.
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Applications for LAMC2 Antibody (CL2980) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LAMC2

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature.

Alternate Names

BM600, BM600-100kDa, Cell-scattering factor 140 kDa subunit, CSF, CSF 140 kDa subunit, EBR2, EBR2A, Epiligrin subunit gamma, kalinin (105kD), Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, kalinin-105kDa, ladsin (140kDa), Ladsin 140 kDa subunit, LAMB2Tlaminin, gamma 2 (nicein (100kD), kalinin (105kD), BM600 (100kD), Herlitzjunctional epidermolysis bullosa)), Laminin B2t chain, laminin subunit gamma-2, laminin, gamma 2, Laminin-5 subunit gamma, LAMNB2B2T, Large adhesive scatter factor 140 kDa subunit, MGC138491, MGC141938, nicein (100kDa), Nicein subunit gamma, nicein-100kDa

Gene Symbol

LAMC2

Additional LAMC2 Products

Product Documents for LAMC2 Antibody (CL2980) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LAMC2 Antibody (CL2980) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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