KLHL8 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04914
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 271-620 of human KLHL8 (NP_065854.3). AKEQIVKQNLKCRDLLDEARNYHLHLSSRAVPDFEYSIRTTPRKHTAGVLFCVGGRGGSGDPFRSIECYSINKNSWFFGPEMNSRRRHVGVISVEGKVYAVGGHDGNEHLGSMEMFDPLTNKWMMKASMNTKRRGIALASLGGPIYAIGGLDDNTCFNDVERYDIESDQWSTVAPMNTPRGGVGSVALVNHVYAVGGNDGMASLSSVERYDPHLDKWIEVKEMGQRRAGNGVSKLHGCLYVVGGFDDNSPLSSVERYDPRSNKWDYVAALTTPRGGVGIATVMGKIFAVGGHNGNAYLNTVEAFDPVLNRWELVGSVSHCRAGAGVAVCSCLTSQIRDVGHGSNNVVDCM
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit KLHL8 Antibody - Azide and BSA Free (NBP3-04914) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for KLHL8 Antibody - Azide and BSA Free
![Western Blot: KLHL8 AntibodyAzide and BSA Free [NBP3-04914]](https://resources.bio-techne.com/images/products/KLHL8-Antibody-Western-Blot-NBP3-04914-img0004.jpg "Western Blot: KLHL8 AntibodyAzide and BSA Free [NBP3-04914]")
Western Blot: KLHL8 AntibodyAzide and BSA Free [NBP3-04914]
Western Blot: KLHL8 Antibody [NBP3-04914] - Analysis of extracts of various cell lines, using KLHL8 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit![Immunocytochemistry/ Immunofluorescence: KLHL8 Antibody - Azide and BSA Free [NBP3-04914]](https://resources.bio-techne.com/images/products/KLHL8-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04914-img0003.jpg "Immunocytochemistry/ Immunofluorescence: KLHL8 Antibody - Azide and BSA Free [NBP3-04914]")
Immunocytochemistry/ Immunofluorescence: KLHL8 Antibody - Azide and BSA Free [NBP3-04914]
Immunocytochemistry/Immunofluorescence: KLHL8 Antibody [NBP3-04914] - Analysis of U-2 OS cells using KLHL8 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunocytochemistry/ Immunofluorescence: KLHL8 Antibody - Azide and BSA Free [NBP3-04914]](https://resources.bio-techne.com/images/products/KLHL8-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04914-img0002.jpg "Immunocytochemistry/ Immunofluorescence: KLHL8 Antibody - Azide and BSA Free [NBP3-04914]")
Immunocytochemistry/ Immunofluorescence: KLHL8 Antibody - Azide and BSA Free [NBP3-04914]
Immunocytochemistry/Immunofluorescence: KLHL8 Antibody [NBP3-04914] - Analysis of L929 cells using KLHL8 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Applications for KLHL8 Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL.
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: KLHL8
Alternate Names
kelch-like 8 (Drosophila), kelch-like protein 8, KIAA1378FLJ46304
Gene Symbol
KLHL8
Additional KLHL8 Products
Product Documents for KLHL8 Antibody - Azide and BSA Free
Product Specific Notices for KLHL8 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov