IRF1 Antibody (BLR039F)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76419

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-76419
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR039F

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IRF1 Antibodies »

Product Specifications

Immunogen

Immunogen sequence between amino acids 250 and 300

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit IRF1 Antibody (BLR039F) (NBP2-76419) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IRF1 Antibody (BLR039F)

Western Blot: IRF1 Antibody (BLR039F) [NBP2-76419]

Western Blot: IRF1 Antibody (BLR039F) [NBP2-76419]

Western Blot: IRF1 Antibody (BLR039F) [NBP2-76419] - Detection of human IRF1 by WB of Jurkat, Hep-G2, HeLa (IFNgamma treated), HeLa, SR, MOLT-4, RPMI-8226, KG-1, A-549, and MCF-7 lysate. Antibody: Rabbit anti-IRF1 recombinant monoclonal [BLR039F] (NBP2-76419). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.
Immunocytochemistry: IRF1 Antibody (BLR039F) [NBP2-76419]

Immunocytochemistry: IRF1 Antibody (BLR039F) [NBP2-76419]

Immunocytochemistry: IRF1 Antibody (BLR039F) [NBP2-76419] - Detection of human IRF1 in FFPE OVCAR-8 cells by ICC. Antibody: Rabbit anti-IRF1 recombinant monoclonal [BLR039F] (NBP2-76419). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry: IRF1 Antibody (BLR039F) [NBP2-76419]

Immunohistochemistry: IRF1 Antibody (BLR039F) [NBP2-76419]

Immunohistochemistry: IRF1 Antibody (BLR039F) [NBP2-76419] - Detection of human IRF1 in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-IRF1 recombinant monoclonal [BLR039F] (NBP2-76419). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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Applications for IRF1 Antibody (BLR039F)

Application
Recommended Usage

Flow Cytometry

Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl

Immunocytochemistry/ Immunofluorescence

1:100 to 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 to 1:500

Immunoprecipitation

20 ul/1mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: IRF1

Interferon regulatory factor 1 (IRF1) is a member of the IRF family of transcription factors that plays a critical role in immune cell function and development. In addition to binding upstream regulatory regions of the type I IFN gene, IRF1 also regulates expression of MHC lass I, and plays a role in apoptosis.

Long Name

Interferon Regulatory Factor 1

Alternate Names

MAR

Gene Symbol

IRF1

Additional IRF1 Products

Product Documents for IRF1 Antibody (BLR039F)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IRF1 Antibody (BLR039F)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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