Integrin alpha 6/CD49f Antibody (450-30A) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-63902

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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 450-30A

Concentration

0.1 mg/ml

View all Integrin alpha 6/CD49f Antibodies »

Product Specifications

Immunogen

alpha 6 beta 4 integrin purified from A431 cells

Specificity

Recognizes CD49f, also known as VLA-6 and alpha 6 integrin. CD49f is expressed by platelets, weakly by monocytes and by a subset of lymphocytes. CD49f is also widely expressed on epithelial tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Integrin alpha 6/CD49f Antibody (450-30A) [FITC]

Western Blot: Integrin alpha 6/CD49f Antibody (450-30A) [FITC] [NB100-63902] - Analysis of Jurkat whole cell lysate.
Flow Cytometry: Integrin alpha 6/CD49f Antibody (450-30A) [FITC] [NB100-63902] - Staining of human peripheral blood monocytes.
Flow Cytometry: Integrin alpha 6/CD49f Antibody (450-30A) [FITC] [NB100-63902] - Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD49f:FITC
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Applications for Integrin alpha 6/CD49f Antibody (450-30A) [FITC]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1% Bovine Serum Albumin

Preservative

0.09% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 6/CD49f

Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Integrin alpha 6 complexes are receptors for laminins, which are components of basement membranes. Integrin alpha 6 complexes may play an important role in embryogenesis.

Alternate Names

CD49f, ITGA6, Platelet gpl, VLA-6

Gene Symbol

ITGA6

UniProt

P23229

Additional Integrin alpha 6/CD49f Products

Product Documents for Integrin alpha 6/CD49f Antibody (450-30A) [FITC]

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin alpha 6/CD49f Antibody (450-30A) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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