Influenza A H1N1 Hemagglutinin Antibody (107) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89960
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Influenza A Virus H1N1
Applications
ELISA, Sandwich ELISA Detection, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 107
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Influenza A H1N1 Hemagglutinin (Accession#: ABD77675.1; Met 1-Gln 528).
Reactivity Notes
Influenza A H1N1
Specificity
No cross-reactivity in ELISA with: H1N1 (A/Brevig Mission/1/1918) HAH1N1 (A/Brisbane/02/2018) HAH1N1 (A/California/04/2009) HAH1N1 (A/California/07/2009) HAH1N1 (A/Guangdong-Maonan/SWL1536/2019) / (A/Hawaii/70/2019) HAH1N1 (A/Michigan/45/2015) HAH1N1 (A/New Caledonia/20/1999) HAH1N1 (A/Wisconsin/588/2019) / (A/Victoria/2570/2019) HAH2N2 (A/Canada/720/2005) HAH3N2 (A/Brisbane/10/2007) HAH3N2 (A/Cambodia/e0826360/2020) HAH3N2 (A/Hong Kong/2671/2019) HAH3N2 (A/Hong Kong/45/2019) HAH3N2 (A/Hong Kong/4801/2014) HAH3N2 (A/Kansas/14/2017) HAH3N2 (A/Singapore/INFIMH-16-0019/2016) HAH3N2 (A/Switzerland/9715293/2013) HAH4N4 (A/mallard/Ohio/657/2002) HAH5N1 (A/Anhui/1/2005) HAH6N1 (A/northern shoveler/California/HKWF115/2007) HAH7N7 (A/chicken/Netherlands/1/03) HAH8N4 (A/pintail duck/Alberta/114/1979) HAH9N2 (A/Hong Kong/1073/99) HAH10N3 (A/duck/Hong Kong/786/1979) HAH11N2 (A/duck/Yangzhou/906/2002) HAH12N5 (A/green-winged teal/ALB/199/1991) HAH13N8 (A/black-headed gull/Netherlands/1/00) HAH15N8 (A/duck/AUS/341/1983) HAH16N3 (A/black-headed gull/Sweden/5/99) HAInfluenza B (B/Brisbane/60/2008) HAInfluenza B (B/Colorado/06/2017) HAInfluenza B (B/Florida/4/2006) HAInfluenza B (B/Florida/4/2006) HAInfluenza B (B/PHUKET/3073/2013) HAInfluenza B (B/Washington/02/2019) HA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Influenza A H1N1 Hemagglutinin Antibody (107) - Azide and BSA Free
![Influenza A H1N1 Hemagglutinin Antibody (107) Western Blot: Influenza A H1N1 Hemagglutinin Antibody (107) [NBP2-89960]](https://resources.bio-techne.com/images/products/nbp2-89960_rabbit-influenza-a-h1n1-hemagglutinin-mab-107-45202316595428.png "Western Blot: Influenza A H1N1 Hemagglutinin Antibody (107) [NBP2-89960]")
Western Blot: Influenza A H1N1 Hemagglutinin Antibody (107) [NBP2-89960]
NBP2-89960 used at 1:1000 dilution. Lane A: H3N2 (A/Darwin/9/2021) HA Protein. Lane B: H3N2 (A/Darwin/9/2021) HA Protein. Lane C: H3N2 (A/Darwin/6/2021) HA Protein. Lane D: Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA Protein. Lane E: Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA Protein. Secondary Goat Anti-Rabbit IgG (H+L)[HRP] used at at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.Applications for Influenza A H1N1 Hemagglutinin Antibody (107) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Sandwich ELISA Detection
1:1000-1:10000
Western Blot
1:500-1:2000
Application Notes
This antibody can be used with the appropriate secondary reagents to detect Influenza A H1N1 Hemagglutinin - (A/Puerto Rico/8/1934) HA in Western Blot. Using a DAB detection system, the detection limit for Influenza A H1N1 Hemagglutinin - (A/Puerto Rico/8/1934) is approximately 5 ng/lane under non-reducing conditions and reducing conditions. The paired capture antibody is NBP2-89959.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Influenza A H1N1 Hemagglutinin
Additional Influenza A H1N1 Hemagglutinin Products
Product Documents for Influenza A H1N1 Hemagglutinin Antibody (107) - Azide and BSA Free
Product Specific Notices for Influenza A H1N1 Hemagglutinin Antibody (107) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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