IgG2c Antibody (RM223) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25957
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM223 expressed in HEK293
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Mouse IgG2c
Specificity
This antibody reacts to the Fc region of mouse IgG2c.No cross reactivity with mouse IgG1, IgG2a, IgG2b,IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit IgG2c Antibody (RM223) - Azide and BSA Free (NBP2-62013) is a recombinant monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for IgG2c Antibody (RM223) - Azide and BSA Free
ELISA: IgG2c Antibody (RM223) - Azide and BSA Free [NBP3-25957] -
ELISA: IgG2c Antibody (RM223) - Azide and BSA Free [NBP3-25957] - A titer ELISA of Mouse IgG2c. The plate was coated with different amounts of Mouse IgG2c. A serial dilution of RM223 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.Western Blot: IgG2c Antibody (RM223) - Azide and BSA Free [NBP3-25957] -
Western Blot: IgG2c Antibody (RM223) - Azide and BSA Free [NBP3-25957] - Western blot of nonreduced(-) and reduced(+) Mouse IgG2c, using Clone RM223. This antibody reacts to nonreduced IgG2c.ELISA: IgG2c Antibody (RM223) - Azide and BSA Free [NBP3-25957] -
ELISA: IgG2c Antibody (RM223) - Azide and BSA Free [NBP3-25957] - ELISA of IgG from BalB/C and C57BL6 shows RM223 reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM223 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.Applications for IgG2c Antibody (RM223) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.01 ug/ml - 0.2 ug/ml
Western Blot
0.5 ug/ml-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG2c
Additional IgG2c Products
Product Documents for IgG2c Antibody (RM223) - Azide and BSA Free
Product Specific Notices for IgG2c Antibody (RM223) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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