IgG1 Antibody (RM117)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62028
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM117 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Peptide corresponding to the hinge region of Human IgG1
Specificity
This antibody reacts to the heavy chain of human IgG1. It does not cross react to any other IgG subclasses (IgG2, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, IgE. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit IgG1 Antibody (RM117) (NBP2-62028) is a recombinant monoclonal antibody validated for use in IHC, ELISA, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for IgG1 Antibody (RM117)
Sandwich ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP2-62028] -
Sandwich ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP2-62028] - Sandwich ELISA using RM117 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an AP conjugated streptavidin.
ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP2-62028] -
ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP2-62028] - ELISA of human immunoglobulins shows RM117 only reacts to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP2-62028] -
ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP2-62028] - A titer ELISA using RM117. The plate was coated with different amounts of human IgG1. A serial dilution of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Applications for IgG1 Antibody (RM117)
Application
Recommended Usage
Flow Cytometry
2ug/ml-8ug/ml
Immunocytochemistry/ Immunofluorescence
0.5ug/ml - 2ug/ml
Immunohistochemistry
0.5ug/ml - 2ug/ml
Immunohistochemistry-Paraffin
0.5ug/ml - 2ug/ml
Sandwich ELISA Capture
50 - 200ng/well
Sandwich ELISA Detection
0.05 - 0.2ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG1
Long Name
Immunoglobulin G1
Alternate Names
IgG1, Igh-4, RGD1359539, VH7183
Additional IgG1 Products
Product Documents for IgG1 Antibody (RM117)
Product Specific Notices for IgG1 Antibody (RM117)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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