IFIT1 Antibody (OTI3G8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02340

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3G8

Concentration

1 mg/ml

View all IFIT1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IFIT1 (NP_000899) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

55.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IFIT1 Antibody (OTI3G8)

Immunocytochemistry/ Immunofluorescence: IFIT1 Antibody (OTI3G8) [NBP2-02340]

Immunocytochemistry/ Immunofluorescence: IFIT1 Antibody (OTI3G8) [NBP2-02340]

Immunocytochemistry/Immunofluorescence: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT1.
Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340]

Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340]

Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of paraffin-embedded Human liver tissue using anti-IFIT1 mouse monoclonal antibody.
Flow Cytometry: IFIT1 Antibody (OTI3G8) [NBP2-02340]

Flow Cytometry: IFIT1 Antibody (OTI3G8) [NBP2-02340]

Flow Cytometry: IFIT1 Antibody (OTI3G8) [NBP2-02340] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IFIT1 antibody, and then analyzed by flow cytometry.
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Applications for IFIT1 Antibody (OTI3G8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:500-1000
Application Notes
IHC-P, we recommend using Tris/EDTA pH 8.0 heat induced epitope retrieval.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IFIT1

IFIT1, also known as C56 and ISG56, is an interferon-induced protein. Its expression can be activated by all three types of interferon in human fibroblasts and lymphoblastoid cells. ISG56 is believed to be a mediator of negative-feedback regulation of virus-triggered induction of type I interferons and cellular antiviral responses.

Alternate Names

G10P1C56, GARG-16, IFI56IFI-56, IFI-56K, IFIT-1, IFNAI1ISG56, Interferon, alpha-inducible protein (MW 56kD), Interferon-induced 56 kDa protein, interferon-induced protein 56, interferon-induced protein with tetratricopeptide repeats 1, p56, RNM561

Entrez Gene IDs

3434 (Human)

Gene Symbol

IFIT1

Additional IFIT1 Products

Product Documents for IFIT1 Antibody (OTI3G8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IFIT1 Antibody (OTI3G8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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