Detects human Vitronectin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant bovine vitronectin or recombinant mouse vitronectin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human Vitronectin Antibody

Detection of Human Vitronectin by Western Blot.

Western blot shows human serum. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Vitronectin Monoclonal Antibody (Catalog # MAB2349) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Vitronectin at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Vitronectin in Human Bladder.

Vitronectin was detected in immersion fixed paraffin-embedded sections of human bladder using Mouse Anti-Human Vitronectin Monoclonal Antibody (Catalog # MAB2349) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Vitronectin by Simple Western^TM.

Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for Vitronectin at approximately 85 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Vitronectin Monoclonal Antibody (Catalog # MAB2349). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Applications for Human Vitronectin Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human bladder and breast

Simple Western

5 µg/mL
Sample: Human serum

Western Blot

0.1 µg/mL
Sample: Human serum

Reviewed Applications

Read 2 reviews rated 4.5 using MAB2349 in the following applications:

ELISA (1 Review)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vitronectin

Vitronectin is a 71 kDa secreted glycoprotein produced by the liver and tumor cells. In blood, Vitronectin is called serum spreading factor. In the extracellular matrix, its function is determined by binding partners such as PAI-1, complement factors, integrins (notably alpha_v beta₃) and thrombin. The 459 aa mature human Vitronectin shows 74% amino acid identity with mouse Vitronectin and contains somatomedin B-like and hemopexin-like domains, an RGD motif, a basic heparin-binding domain and sulfated tyrosines. Unbound Vitronectin is a monomer that may be cleaved to form a dimer of 65 kDa and 10 kDa components.

Alternate Names

Complement S-protein, Serum Spreading Factor, Somatomedin B, VTN

Entrez Gene IDs

7448 (Human); 22370 (Mouse); 507525 (Bovine)

Gene Symbol

VTN

Additional Vitronectin Products

Product Documents for Human Vitronectin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Vitronectin Antibody

For research use only

Citations
Reviews

Human Vitronectin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2349

Key Product Details

Species Reactivity

Validated:

Cited:

Applications