Detects human ULBP‑4/RAET1E in direct ELISAs and Western blot. In Western blot, approximately 20% cross‑reactivity with recombinant human (rh) ULBP-2 is observed and no cross-reactivity with rhULBP‑1 or rhULBP-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human ULBP-4/RAET1E PE-conjugated Antibody

Detection of ULBP-4/RAET1E in HEK293 cells by Flow Cytometry

HEK293 human embryonic kidney cells transfected with (A) ULBP-4/RAET1E or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human ULBP-4/RAET1E PE-conjugated Monoclonal Antibody (Catalog # FAB6285P). View our protocol for Staining Membrane-associated Proteins

Applications for Human ULBP-4/RAET1E PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HEK293 cells transfected with Human ULBP‑4/RAET1E

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ULBP-4/RAET1E

ULBP-4 (cytomegalovirus glycoprotein UL16 binding protein 4), also called RAET1E (retinoic acid early transcript 1E), Letal (lymphocyte effector cell toxicity activating ligand) and NKG2DL4 (NKG2D ligand 4), is a 40-50 kDa member of the ULBP/RAET1 family of cell surface proteins that function as ligands for NKG2D (1‑6). While most family members are GPI-anchored, only ULBP-4/RAET1E and ULBP-5/RAET1G express a transmembrane form (1, 4, 6, 7). Human ULBP-4 mRNA encodes 263 amino acids (aa) including a 30 aa signal sequence, a 195 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 15 aa cytoplasmic sequence. A soluble 35 kDa form diverges at aa 208 and is thought to antagonize the transmembrane form (5). Other potential splice variants of 220, 227 and 280 aa are transmembrane proteins (8). Within the ECD, ULBP-4 shares 34-41% aa sequence identity with family members (1, 7). Rodent NKG2D ligands Rae-1 alpha-epsilon are, like human ULBP and MIB proteins, distantly related to MHC class I proteins, but none of the families share significant sequence identity (2, 4). Low expression of ULBP‑4 mRNA is found in normal tissues, with high expression variably reported in the small intestine (3) and skin (4). Expression is stimulated by TNF-alpha and down‑regulated by retinoic acid (3). ULBP-4 is abnormally expressed on most colon cancer and some other tumor cell lines and virus-infected peripheral blood cells (3, 6). ULBP-4 binds and costimulates NKG2D-expressing effector cells including NK cells, NKT cells, gamma delta T cells, and CD8⁺ alpha beta T cells, activating cytolytic activity and/or cytokine production (3, 4, 7). In some gamma delta T cells, direct ULBP-4 binding to both TCR gamma delta and NKG2D has been demonstrated (6). ULBP-4 is also thought to function as a minor histocompatibility antigen in humans (1).

References

Radosavljevic, M. et al. (2002) Genomics 79:114.
Kondo, M. et al. (2010) Immunogenetics 62:441.
Conejo-Garcia, J.R. et al. (2003) Cancer Biol. Ther. 2:446.
Chalupny, N.J. et al. (2003) Biochem. Biophys. Res. Commun. 305:129.
Cao, W. et al. (2007) J. Biol. Chem. 282:18922.
Kong, Y. et al. (2009) Blood 114:310.
Bacon, L. et al. (2004) J. Immunol. 173:1078.
Cao, W. et al. (2008) Int. Immunol. 20:981.

Long Name

UL16 Binding Protein-4

Alternate Names

LETAL, N2DL-4, N2DL4, NKG2DL4, RAET1E, RL-4, ULBP4

Entrez Gene IDs

135250 (Human); 379043 (Mouse); 292461 (Rat)

Gene Symbol

RAET1E

UniProt

Q8TD07 (Human)

Additional ULBP-4/RAET1E Products

Product Documents for Human ULBP-4/RAET1E PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ULBP-4/RAET1E PE-conjugated Antibody

For research use only

Citations
Reviews

Human ULBP-4/RAET1E PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6285P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all ULBP-4/RAET1E Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ULBP-4/RAET1E PE-conjugated Antibody

Detection of ULBP-4/RAET1E in HEK293 cells by Flow Cytometry

Applications for Human ULBP-4/RAET1E PE-conjugated Antibody

Flow Cytometry

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: ULBP-4/RAET1E

References

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional ULBP-4/RAET1E Products

Product Documents for Human ULBP-4/RAET1E PE-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Human ULBP-4/RAET1E PE-conjugated Antibody