Human Tyro3/Dtk Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB859
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Knockout Validated, Immunohistochemistry, ELISA Capture (Matched Antibody Pair), Flow Cytometry
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Bioassay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 96201
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tyro3/Dtk
Ala41-Ser428 (predicted)
Accession # Q06418
Ala41-Ser428 (predicted)
Accession # Q06418
Specificity
Detects human Tyro3/Dtk in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity or interferance with recombinant mouse Tyro3/Dtk, recombinant human (rh) Axl, or rhMer is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Tyro3/Dtk Antibody
Detection of Dtk in K562 Human Cell Line by Flow Cytometry.
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Dtk Monoclonal Antibody (Catalog # MAB859, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Tyro3/Dtk Specificity is Shown by Flow Cytometry in Knockout Cell Line.
Tyro3/Dtk knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human Tyro3/Dtk Monoclonal Antibody (Catalog # MAB859, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the Tyro3/Dtk knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Detection of Tyro3/Dtk by Flow Cytometry
The expression of Tyro3TK on CD14+CD16− monocytes is increased in RA. a Gating strategy for identifying the expression of Tyro3TK on CD14+CD16+ and CD14+CD16− monocytes. Accordingly, the expression of Tyro3TK on CD14+CD16+ and CD14+CD16− monocytes in HC (n = 40) (b), OA (n = 28) (c), and RA patients (n = 40, **P = 0.008) (d) were analyzed and presented as the mean fluorescence intensity (MFI). e The expression of Tyro3TK on CD14+CD16+ monocytes were compared between HC, OA, and RA patients. f The expression of Tyro3TK on CD14+CD16− monocytes were compared between HC, OA, and RA patients (**P = 0.004, ***P < 0.001). g Flow cytometry-sorted CD14+CD16− monocytes from RA (n = 4) and HC (n = 4) were set to detect the mRNA expression of Tyro3TK by qPCR (*P = 0.029). *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant (Mann-Whitney U test, b, d, and g; Student’s t test, c; Kruskal-Wallis test followed by Dunn’s post-test for multiple comparisons, e–f) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32958023), licensed under a CC-BY license. Not internally tested by R&D Systems.
Applications for Human Tyro3/Dtk Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Knockout Validated
Tyro3/Dtk is specifically detected in K562 myelogenous leukemia parental cell line but is not detectable in Tyro3/Dtk knockout K562 cell line.
Human Tyro3/Dtk Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tyro3/Dtk
Dtk (also called Sky, Tyro3, Rse, Brt), Axl (Ufo, Ark) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.
References
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Tyrosine Protein Kinase Receptor TYRO3
Alternate Names
Brt, Dtk, Rse, Sky
Gene Symbol
TYRO3
UniProt
Additional Tyro3/Dtk Products
Product Documents for Human Tyro3/Dtk Antibody
Product Specific Notices for Human Tyro3/Dtk Antibody
For research use only
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