Detects human TRAIL R2/TNFRSF10B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhOPG, rhRANK, rhRELT, rhTNF RI, rhTNF RII, rhTRAIL R1, rhTRAIL R3, rhTRAIL R4, or rhTWEAK R is observed. Additionally in direct ELISAs, no cross‑reactivity with rhTRAIL R4, rhXEDAR, recombinant mouse (rm) DR5, rhTRAIL R1, rmTRAIL R2, or rmTNFRH3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human TRAIL R2/TNFRSF10B Antibody

Detection of Human TRAIL R2/TNFRSF10B by Western Blot.

Western blot shows lysates of HCT-116 human colorectal carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TRAIL R2/TNFRSF10B Monoclonal Antibody (Catalog # MAB6313) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for TRAIL R2/TNFRSF10B at approximately 44 and 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human TRAIL R2/TNFRSF10B Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HCT‑116 human colorectal carcinoma cell line and HepG2 human hepatocellular carcinoma cell line

Reviewed Applications

Read 1 review rated 4 using MAB6313 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAIL R2/TNFRSF10B

Human TRAIL R2, also known as DR5 and TRICK 2, is a type 1 membrane protein of the TNF R family, designated TNFRSF10B, which is a receptor for TRAIL (APO2 ligand). The human TRAIL R2 cDNA encodes a 440 amino acid (aa) precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% aa sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

References

Chaudhary, P.M. et al. (1997) Immunity 7:821.
Walczak, H. et al. (1997) EMBO J. 16:5386.
Golstein, P. (1997) Curr. Biol. 7:R750.

Long Name

TRAIL Receptor 2

Alternate Names

CD262, DR5, TNFRSF10B, TRAILR2

Entrez Gene IDs

8795 (Human); 21933 (Mouse)

Gene Symbol

TNFRSF10B

UniProt

NP_003833

Additional TRAIL R2/TNFRSF10B Products

Product Documents for Human TRAIL R2/TNFRSF10B Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human TRAIL R2/TNFRSF10B Antibody

For research use only

Citations
Reviews

Human TRAIL R2/TNFRSF10B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6313

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity